Click-hydrogel delivered aggregation-induced emissive nanovesicles for simultaneous remodeling and antibiosis of deep burn wounds  

作　　者：Xu Chen Meijiao Zhao Qihu Xie Sitong Zhou Xiaoping Zhong Judun Zheng Ronghua Yang Xianjin Du Jinyu Xia Yuhui Liao 

机构地区：[1]Department of Infectious Diseases,The Fifth Affiliated Hospital,Sun Yat-sen University,Zhuhai,China [2]Molecular Diagnosis and Treatment Center for Infectious Diseases,Dermatology Hospital,Southern Medical University,Guangzhou,China [3]Department of Burns and Plastic Surgery,The Second Affiliated Hospital of Shantou University Medical College,Shantou,China [4]Department of Dermatology,The,First People’s Hospital of Foshan,Foshan,China [5]Department of Burn and Plastic Surgery,Guangzhou First People’s Hospital,South China University of Technology,Guangzhou,China [6]Department of Critical Care Medicine,Renmin Hospital of Wuhan University,Wuhan,China

出　　处：《Aggregate》2024年第1期244-256,共13页聚集体（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:82102256,82272276,81972019,82102444,88241059,82272281;Basic and Applied Basic Research Foundation of Guangdong Province,Grant/Award Numbers:2023A1515012375,2021B1515120036,2021A1515011453,2022A1515012160,2021A1515010949;Chinese Postdoctoral Science Foundation,Grant/Award Number:2021M693638;Excellent Young Researchers Program of the 5th Affiliated Hospital of SYSU,Grant/Award Number:WYYXQN-2021008;National Key Research and Development Program of China,Grant/Award Number:2021YFC2302200;Natural Science Fund of Guangdong Province for Distinguished Young。

摘　　要：As a high-risk trauma,deep burns are always hindered in their repair process by decreased tissue regeneration capacity and persistent infections.In this study,we developed a simultaneous strategy for deep burn wounds treatment using functional nanovesicles with antibacterial and tissue remodeling properties,delivered via a click-chemistry hydrogel.An aggregation-induced emission photosensitizer of 4-(2-(5-(4-(diphenylamino)phenyl)thiophen-2-yl)vinyl)-1-(2-hydroxyethyl)pyridin-1-ium bromide(THB)with excellent photodynamic properties was first prepared,and then combined with readily accessible adipose stem cells-derived nanovesicles to generate the THB functionalized nanovesicles(THB@ANVs).The THB@ANVs showed strong antibacterial activity against Gram-positive bacteria(up to 100%killing rate),and also beneficial effects on tissue remodeling,including promoting cell migration,cell proliferation,and regulating immunity.In addition,we prepared a click-hydrogel of carboxymethyl chitosan for effective delivery of THB@ANVs on wounds.This hydrogel could be injected to conform to the wound morphology while responding to the acidic microenvironment.In vivo evaluations of wound healing revealed that the THB@ANVs hydrogel dressing efficiently accelerated the healing of second-degree burn wounds by reducing bacterial growth,regulating inflammation,promoting early angiogenesis,and collagen deposition.This study provides a promising candidate of wound dressing with diverse functions for deep burn wound repair.

关 键 词：aggregation-induced emission burn wounds NANOVESICLES 

分 类 号：O62[理学—有机化学]