白术提取物对LPS诱导BV2细胞抗神经炎症作用  

Anti-neuroinflammatory effects of extracts from rhizomes of Atractylodes macrocephala Koidzumi in LPS-induced BV2 microglial cells

作　　者：李静 金倫喆 金洪光[3] LI Jing;KIM Youn-Chul;JIN Hong-guang(Department of Pharmacy,Jiujiang University Affiliated Hospital,Jiujiang 332000,China;不详)

机构地区：[1]九江学院附属医院药剂科,江西九江332000 [2]韩国圆光大学药学院,韩国益山54538 [3]九江学院,江西九江332000

出　　处：《中国处方药》2024年第2期60-64,共5页Journal of China Prescription Drug

基　　金：江西省教育厅科学技术青年项目(GJJ211841);江西省卫生健康委科技计划(SKJP220219734);江西省中医药管理局科技计划(2021Z017)。

摘　　要：目的研究白术甲醇-正己烷层萃取物(AH)对脂多糖(LPS)诱导的BV2细胞抗神经炎症作用及其相关机制,并分析AH主要成分。方法制备白术提取物;以高效液相色谱法(HPLC)分析AH主要成分;以LPS诱导BV2细胞建立炎症模型;以四甲基偶氮唑蓝(MTT)法检测细胞存活率;以Griess反应检测细胞上清液中NO水平;以ELISA法检测细胞上清液中前列腺素E_(2)(PGE_(2))、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平;以Western Blot法检测环氧合酶-2(COX-2)、诱导型一氧化氮合酶(iNOS)、丝裂原活化蛋白激酶(MAPK)、核转录因子-κB(NF-κB)蛋白表达。结果AH主要成分包括白术内酯Ⅱ、Ⅲ。AH可显著降低LPS诱导BV2细胞产生的NO、PGE_(2)、IL-6、TNF-α水平,同时降低iNOS、COX-2、MAPK、NF-κB蛋白表达。低浓度AH对PGE_(2)的抑制作用不弱于高浓度白术内酯Ⅱ、Ⅲ;高浓度AH对NO的抑制作用与高浓度白术内酯Ⅲ相似。结论AH回收率高,主要成分包括白术内酯Ⅱ、Ⅲ;AH有较好的抗神经炎症作用,其抗炎机制与MAPK和NF-κB通路有关。Objective The research is to investigate the anti-neuroinflammatory effects and related mechanism of methanol-n-Hexane extracts from rhizomes of Atractylodes macrocephala Koidzumi(AH)in LPS-induced BV2 microglial cells,and analyze the main components of the AH.Methods The extracts from rhizomes of Atractylodes macrocephala Koidzumi were made.The main components of the AH was analyzed by highperformance liquid chromatography(HPLC).BV2 microglial cells were induced by LPS to establish an inflammatory model.MTT assay was used to test the cell viability.The content of NO in cell supernatant was measured by Griess reagent.The levels of PGE_(2),IL-6,and TNF-αwere measured by ELISA kits.The expression of COX-2,iNOS,MAPK and NF-κB proteins were detected by Western blot.Results The main components of AH included atractylenolideⅡandⅢ.AH significantly reduced the levels of NO,PGE_(2),IL-6,and TNF-αproduced by LPS-induced BV2 microglial cells,while reduced iNOS,COX-2,MAPK and NF-κB proteins expression.Additionally,the inhibitory effect of low concentration AH on PGE_(2)was not weaker than that of high concentration of atractonolideⅡandⅢ,and the inhibitory effect of high concentration AH was similar with high concentration atractylenolideⅢ.Conclusion The recovery of AH is high,and the main components of AH include atractylenolideⅡandⅢ.AH has a better anti-neuroinflammatory effect,and its anti-inflammatory mechanism is related to the MAPK and NF-κB pathways.

关 键 词：白术 白术内酯Ⅱ 白术内酯Ⅲ 神经炎症 

分 类 号：R285[医药卫生—中药学]