MicroRNA调控在溶瘤病毒疗法中的应用与展望  

Application of MicroRNA Regulation in Oncolytic Virotherapy and Its Prospects

作　　者：赵灿阳 林燕真[2] 程通[3] 王玮 ZHAO Canyang;LIN Yanzhen;CHENG Tong;WANG Wei(National Institute of Diagnostics and Vaccine Development in Infectious Diseases,School of Pulic Health,Xiamen University,Xiamen 361102,China;Affiliated Zhongshan Hospital,Xiamen University,Xiamen 361004,China;School of Life Sciences,Xiamen University,Xiamen 361102,China)

机构地区：[1]厦门大学公共卫生学院,国家传染病诊断试剂与疫苗工程技术研究中心,厦门361102 [2]厦门大学附属中山医院,厦门361004 [3]厦门大学生命科学学院,厦门361102

出　　处：《中国生物工程杂志》2024年第1期98-106,共9页China Biotechnology

基　　金：福建省自然科学基金(2022J011352)资助项目。

摘　　要：MicroRNA(miRNA)是一类内源性的、能够特异性调控真核生物细胞内转录后基因表达的非编码单链RNA。近年来,miRNA的调控作用已被广泛应用于溶瘤病毒的设计。一方面,通过在病毒基因组中插入肿瘤内表达下调的组织特异性miRNA的靶序列,可以改变溶瘤病毒的组织趋向性,在达到减毒效果的同时保留其抗肿瘤活性。另一方面,通过在病毒基因组中插入表达天然或人工改造的miRNA,可以促进病毒复制,改善肿瘤微环境,从而增强溶瘤病毒的疗效。讨论目前miRNA调控在溶瘤病毒领域中的应用进展,为优化溶瘤病毒疗法策略提供参考。MicroRNAs(miRNAs)are a type of endogenous,non-coding,single-stranded RNA that specifically regulates post-transcriptional gene expression in eukaryotic cells.In recent years,miRNA regulation has been utilized in the design of oncolytic viruses.By inserting a target sequence of a tissue-specific miRNA that is down-regulated in the tumor into the viral genome,the tissue tropism of the oncolytic virus can be altered,and the anti-tumor activity of the virus can be remained during viral attenuation.Additionally,natural or artificially modified miRNAs can be inserted and expressed in viral genomes to promote viral replication and improve the tumor microenvironment,thereby enhancing the therapeutic effect of oncolytic viruses.This paper reviews and discusses the utilization of miRNA regulation in oncolytic virology,with the aim of providing insights into the optimization of existing oncolytic virotherapy strategies.

关 键 词：MicroRNA 溶瘤病毒 靶向调控 病毒治疗 

分 类 号：Q789[生物学—分子生物学]