茶谷蛾触角气味结合蛋白基因分析  

Transcriptome Sequencing Analysis of Odorant Binding Protein Genes in Antennae of Agriophara rhombata Meyr.

作　　者：曲浩 王雪松[1] 罗梓文[1] 王凯博 龙亚芹[1] 陈林波[1] QU Hao;WANG Xuesong;LUO Ziwen;WANG Kaibo;LONG Yaqin;CHEN Linbo(Yunnan Provincial Key Laboratory of Tea Science,Yunnan Technology Engineering Research Center of Tea Germplasm Innovation and Supporting Cultivation,Tea Research Institute,Yunnan Academy of Agricultural Sciences,Kunming,650205)

机构地区：[1]云南省农业科学院茶叶研究所,云南省茶树种质资源创新与配套栽培技术工程研究中心,云南省茶学重点实验室,昆明650205

出　　处：《基因组学与应用生物学》2024年第1期100-108,共9页Genomics and Applied Biology

基　　金：云南省农业联合专项重点项目(202101BD07001-029);国家自然科学基金地区项目(32160724)共同资助。

摘　　要：茶谷蛾(Agriophara rhombata Meyr.)触角上表达的嗅觉相关基因是其交配、寻找食物来源、寻找产卵地、避免有害化合物等行为的重要分子基础。为了研究茶谷蛾触角的嗅觉机制,分析了雌雄蛾触角功能差异,并筛选触角气味结合蛋白基因,本研究采用转录组测序和实时定量PCR技术,对茶谷蛾雌、雄蛾触角的基因表达情况进行了分析,共注释到37666个基因,其中雌、雄蛾共有的表达基因数目为34720个,雌蛾特有表达基因有1739个,雄蛾特有表达基因有1207个。在雌、雄蛾触角中共鉴定到170个化学感受相关基因,包括33个气味结合蛋白基因(odorant binding protein genes,OBPs),42个味觉受体基因(gustatory receptor genes,GRs),12个化学感受蛋白基因(chemosensory protein genes,CSPs),52个嗅觉受体基因(olfactory receptor genes,ORs),27个离子型受体基因(ionotropic receptor genes,IRs),4个感觉神经元膜蛋白基因(sensory neuron membrane protein genes,SNMPs)。OBP蛋白基因中16个聚集在信息素结合蛋白基因(pheromone-binding protein genes,PBPs)亚家族,14个聚集在普通气味结合蛋白基因(general odorant-binding protein genes,GOBPs)亚家族,3个聚集在触角结合蛋白基因(antennal binding protein genes,ABPs)亚家族。本研究为茶谷蛾嗅觉分子机制研究提供了线索,为茶谷蛾嗅觉机制的研究以及茶谷蛾绿色防控药剂的开发奠定了基础。The olfactor-related genes expressed on the antennae of tea millet moth(Agrophara rhombata Meyr.)are the important molecular basis for mating,finding food sources,finding spawning sites,and avoiding harmful compounds.In order to study the olfactory mechanism of antennae of tea millet moth,gene expression in the antennae of female and male moth were analyzed by transcriptome sequencing technology and real time quantitative PCR.A total of 37666 genes were annotated.The number of expression genes shared by the female and male moths was 34720,1739 specific expression genes of the female moths and 1207 specific expression genes of the male moths.A total of 170 chemoreception-related genes were identified in the antennae of female and male moths,including 33 odorant binding protein genes(OBPs),42 gustatory receptor genes(GRs),12 chemosensory protein genes(CSPs),52 olfactory receptor genes(ORs),27 ionotropic receptor genes(IRs),and 4 sensory neuron membrane protein genes(SNMPs).Among OBPs,16 clustered in pheromone-binding protein genes(PBPs)subfamily,14 clustered in general odorant-binding protein genes(GOBPs)subfamily,3 clustered in the antennal binding protein genes(ABPs)subfamily.This study provides clues for the molecular mechanism of the olfactory sense of the Agrophara rhombata Meyr.,and lay a foundation for the research of the olfactory mechanism of the tea millet moth and the development of green control agents for the tea millet moth.

关 键 词：茶谷蛾 触角 转录组 嗅觉相关基因 气味结合蛋白基因 

分 类 号：S433[农业科学—农业昆虫与害虫防治]