Genome assembly of Polygala tenuifolia provides insights into its karyotype evolution and triterpenoid saponin biosynthesis  

作　　者：Fanbo Meng Tianzhe Chu Pengmian Feng Nan Li Chi Song Chunjin Li Liang Leng Xiaoming Song Wei Chen 

机构地区：[1]State Key Laboratory of Southwestern Chinese Medicine Resources,School of Basic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China [2]State Key Laboratory of Southwestern Chinese Medicine Resources,Innovative Institute of Chengdu University of Traditional Chinese Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China [3]School of Life Sciences,North China University of Science and Technology,Tangshan 063210,China [4]Institute of Herbgenomics,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China

出　　处：《Horticulture Research》2023年第9期1-12,共12页园艺研究（英文）

基　　金：supported by the Natural Science Foundation of Sichuan(No.2023NSFSC0683);Innovation Team and Talents Cultivation Program of the National Administration of Traditional Chinese Medicine(No:ZYYCXTD-D-202209);the‘Xinglin Scholar’Discipline Talent Research Promotion Program of Chengdu University of TCM(No.MPRC2021036).

摘　　要：Polygala tenuifolia is a perennial medicinal plant that has been widely used in traditional Chinese medicine for treating mental diseases.However,the lack of genomic resources limits the insight into its evolutionary and biological characterization.In the present work,we reported the P.tenuifolia genome,the first genome assembly of the Polygalaceae family.We sequenced and assembled this genome by a combination of Illumnina,PacBio HiFi,and Hi-C mapping.The assembly includes 19 pseudochromosomes covering∼92.68%of the assembled genome(∼769.62 Mb).There are 36463 protein-coding genes annotated in this genome.Detailed comparative genome anal-ysis revealed that P.tenuifolia experienced two rounds of whole genome duplication that occurred∼39–44 and∼18–20 million years ago,respectively.Accordingly,we systematically reconstructed ancestral chromosomes of P.tenuifolia and inferred its chromosome evolution trajectories from the common ancestor of core eudicots to the present species.Based on the transcriptomics data,enzyme genes and transcription factors involved in the synthesis of triterpenoid saponin in P.tenuifolia were identified.Further analysis demonstrated that whole-genome duplications and tandem duplications play critical roles in the expansion of P450 and UGT gene families,which contributed to the synthesis of triterpenoid saponins.The genome and transcriptome data will not only provide valuable resources for comparative and functional genomic researches on Polygalaceae,but also shed light on the synthesis of triterpenoid saponin.

关 键 词：INSIGHT synthesis evolution 

分 类 号：Q943.2[生物学—植物学]