不同居群艾种质遗传多样性和遗传结构分析  

Genetic Diversity and Population Structure of Different Populations in Artemisia argyi Germplasm

作　　者：许兰杰[1] 杨青 余永亮[1] 安素妨 董薇[1] 梁慧珍[1] XU Lanjie;YANG Qing;YU Yongliang;AN Sufang;DONG Wei;LIANG Huizhen(Institute of Chinese Herbel Medicines,Henan Academy of Agricultural Sciences,Zhengzhou,Henan 450002)

机构地区：[1]河南省农业科学院中药材研究所,河南郑州450002

出　　处：《北方园艺》2024年第2期94-100,共7页Northern Horticulture

基　　金：河南省科技攻关资助项目(222102110466,222102110379);国家现代农业产业技术体系资助项目(CARS-21);河南省农科院自主创新专项基金资助项目(2022ZC64),河南省农科院新兴学科发展专项资助项目(2021XK03,2022XK03);河南省中央引导地方科技发展专项自由探索类资助项目(YDZX20214100001804)。

摘　　要：以12个艾居群和22条SCoT引物为试材,采用NTSYS-pc2.1、Popegen 1.32等分析方法对12个居群的遗传多样性和群体结构特征进行研究,以期为艾新品种选育提供参考依据。结果表明:22条SCoT引物共检测出87个主要等位基因变异,平均每条引物3.95个;多态性信息含量PIC值、Shannon′s多样性指数I值和Nei′s基因多样性指数H平均值分别为0.690、0.483和0.317,表现出较高的遗传多样性。居群每个位点平均等位基因数(Na)、每个位点平均有效等位基因数(Ne)、I和期望杂合度(He)平均值分别为1.444、1.419、0.349、0.238,以安阳汤阴县艾居群Na、I、H值最高。居群遗传相似系数和遗传距离范围为0.795~0.975和0.025~0.229,平均值为0.909和0.097。其中,济源艾居群和黄冈蕲春艾居群的遗传相似系数最高、遗传距离最近。SCoT遗传分化系数(Gst)为0.293,表明群体间的遗传多样性水平较低,9%遗传差异源自群体间。居群间基因流水平(Nm)值为2.022,表明居群间存在一定的基因流,导致居群间的遗传差异小。12个艾居群在0.914处划分4个组群,分别包括8、2、1、1个居群。Twelve populations of Artemisia argyi and 22 SCoT primers were used as test material,the genetic diversity and population structure characteristics of the 12 populations were studied by methods of NTSYS-pc 2.1,Popegen 1.32,in order to provide reference for the breeding of new Ai varieties.The results showed that 87 alleles were detected by 22 SCoT markers,with an average of 3.95 alleles for each primers.The average PIC,I and H were 0.690,0.483 and 0.317 that showed high genetic diversity.The average value of Na,Ne,I and He per locus were 1.444,1.419,0.349 and 0.238,Anyang Tangyin population was the highest.Genetic similarity and the genetic distance were 0.795-0.975 and 0.025-0.229,with average of 0.909 and 0.097.Jiyuan and Huanggang Qichun populations had the highest and nearest genetic similarity coefficient and genetic distance.Gst of SCoT was 0.293,indicated a low level of genetic diversity among populations,and genetic differences for 9% was attributable to inter-population.Nm was 2.022 among 12 populations indicated that gene flow was lower among populations,resulting genetic difference was small among populations.Four classes were clustered at the genetic similarity coefficient of 0.914 including 8,2,1 and 1 population,respectively.

关 键 词：艾 居群 遗传多样性 群体遗传结构 

分 类 号：R282.2[医药卫生—中药学]