RNA干扰技术防控作物真菌病害研究进展  

Research Progress on Prevention and Control of Crop Fungal Diseases by RNA Interference Techniques

作　　者：李爽 闫更轩 田缘 张淑梅[1] 潘钰[1] LI Shuang;YAN Gengxuan;TIAN Yuan;ZHANG Shumei;PAN Yu(Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin,Heilongjiang 150010;College of Life Science,Northeast Forestry University,Harbin,Heilongjiang 150040;College of Food Science,Northeast Agricultural University,Harbin,Heilongjiang 150030)

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010 [2]东北林业大学生命科学学院,黑龙江哈尔滨150040 [3]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《北方园艺》2024年第3期127-136,共10页Northern Horticulture

基　　金：黑龙江省属科研院所科研业务费资助项目(CZKYF2021-2-C031)。

摘　　要：作物真菌病害发生普遍,危害严重,是制约现代农业高质量发展的主要限制因素之一。RNA干扰(RNA interference, RNAi)是真核生物中基于双链RNA(double-stranded RNA,dsRNA)介导的同源mRNA降解、沉默相关基因表达的一种机制。近年来,利用RNA干扰技术防控作物真菌病害是研究热点,并已成功应用于农业生产,展现出良好的应用前景。该研究就作物真菌病害防治现状、RNA干扰技术原理、防控真菌病害机制及其在作物真菌病害防控中研究进展、基于RNA干扰生物技术存在的问题与应用前景进行了综述,旨在为RNA干扰技术在作物真菌病害防治中的广泛应用提供参考依据。Crop fungal diseases were common and serious,which was one of the main limiting factors to the high quality development of modern agriculture.RNA interference(RNAi)was a mechanism of homologous mRNA degradation mediated by double-stranded RNA(dsRNA)and silencing the expression of related genes in eukaryotes.In recent years,using RNA interference technology to prevent and control crop fungal diseases has become a research hotspot,and has been successfully applied to agricultural production,showing agood application prospect.In this study,the current situation of crop fungal disease control,the principle of RNA interference technology,the mechanism of fungal disease control and its research progress in crop fungal disease control,the existing problems and application prospects of RNA interference-based biotechnology were reviewed,in order to provide reference for the extensive application of RNA interference technology in crop fungal disease control.

关 键 词：RNA干扰 作物 真菌病害 研究进展 

分 类 号：Q939.9[生物学—微生物学]