HPLC法测定新乐康片相关药材中8种活性成分含量  

HPLC Determined the Content of 8 Active Ingredients in Xinlekang Tablets

作　　者：邓雨荷 张庆林 杨明武 陈伟[2] 何珺 DENG Yuhe;ZHANG Qinglin;YANG Mingwu;CHEN Wei;HE Jun(School of Pharmacy,Guizhou University,Guiyang 550025,China;Engineering Research Center for the Development and Utilization of Southwest Characteristic Pharmaceutical Biological Resources Ministry of Education,Guiyang 550025,China;Shanxi Taiyuan Pharmaceutical Co.LTD.,Taiyuan 030021,China)

机构地区：[1]贵州大学药学院,贵州贵阳550025 [2]西南特色药用生物资源开发利用教育部工程研究中心,贵州贵阳550025 [3]山西太原药业有限公司,山西太原030021

出　　处：《广州化学》2024年第1期75-78,I0004,共5页Guangzhou Chemistry

摘　　要：建立HPLC法同时测定新乐康片中三味原料药材钩藤、酸枣仁和萝芙木中钩藤碱、异钩藤碱、斯皮诺素、酸枣仁皂苷A、酸枣仁皂苷B、育亨宾、阿义吗啉及利血平等8种活性成分的含量。采用Titank C18(250 mm×4.6mm,5μm)色谱柱,以乙腈-0.4%磷酸水溶液(含0.05%三乙胺)为流动相,梯度洗脱,检测波长204 nm,流速为1.0 mL/min,柱温为30℃。上述三味药材相关成分之间分离度良好,在λ=204 nm条件下,其在分别的线性范围内与响应值之间的线性关系良好(R^(2)≥0.9990);8种活性成分的平均加样回收率98.37%~101.21%,RSD<2%,仪器精密度、供试品溶液的稳定性与方法重复性均良好。建立的HPLC法可一法对钩藤、酸枣仁和萝芙木三味中药材中8种活性成分含量进行测定,为药业在生产新乐康片前期选材上节约了测定时间,提高了生产效率,同时为新乐康片中活性成分HPLC含量测定方法的建立及其质量标准升级提供参考。To establish HPLC method to determine the content of eight active ingredients,namely,alkali,sponine,sinin,sinin A,B,Yohimbin,morpholine and reserpine in Xinlekang tablets.Using Titank C18(250 mm×4.6 mm,5μm)column,acetonitrile-0.4%aqueous phosphoric acid solution(containing 0.05%triethylamine)as mobile phase,gradient elution,detection wavelength of 204 nm,the flow rate is 1.0 mL/min,and the column temperature is 30℃.The separation between the related components of the three medicinal materials was good,in the linear range(R^(2)≥0.9990);the average sample recovery of the eight active components was 98.37%~101.21%,RSD<2%,the precision of the instrument and the stability of the test solution and the method were good.The HPLC method can measure the content of 8 active ingredients in uncintus,jujube kernel and lufumu,for the pharmaceutical industry saves the determination time,improves the production efficiency,and provides the determination method of HPLC content and its quality standard upgrade.

关 键 词：钩藤 酸枣仁 萝芙木 钩藤碱 酸枣仁皂苷A 利血平 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]