天马颗粒剂通过TLR4/NF-κB/Klotho途径调控大肠癌裸鼠移植瘤研究  

Study on Tianma Granule Regulating Colorectal Cancer Model byTLR4/NF-κB/Klotho Pathway

作　　者：姚欣 袁婉婧 何永恒 曾宏亮 王梦涵 孙怡 黄兴瑜 肖佑[2] 谢彪[2] Yao Xin;Yuan Wanjing;He Yongheng;Zeng Hongliang;Wang Menghan;Sun Yi;Huang Xingyu;Xiao You;Xie Biao(Hunan University of Chinese Medicine,Changsha 410208,China;The Second Hospital Affiliated to Hunan University of Chinese Medicine,Changsha 410005,China;The Hospital Affiliated to Hunan Academy of Traditional Chinese Medicine,Changsha 410006,China;Hunan Institute of Traditional Chinese Medicine,Changsha 410031,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学第二附属医院,湖南长沙410005 [3]湖南省中医药研究院附属医院,湖南长沙410006 [4]湖南省中医药研究院,湖南长沙410031

出　　处：《亚太传统医药》2024年第2期11-14,共4页Asia-Pacific Traditional Medicine

基　　金：国家自然科学基金青年基金项目(81704089);湖南省自然科学基金青年基金项目(2018JJ3400);长沙市杰出创新青年计划(kq1707012);湖南省自然科学基金(2021JJ40413);湖南省中医药防治肛肠疾病重点研究室基金资助(湘中医药函〔2020〕51号)。

摘　　要：目的:观察天马颗粒剂对大肠癌裸鼠移植瘤组织中TLR4、NF-κB、Klotho表达的影响。方法:以SPF级裸鼠为研究对象,通过皮下种植HCT116细胞株构建裸鼠移植瘤模型,随机分为3组:对照组、天马颗粒低剂量组、天马颗粒正常剂量组,对照组予生理盐水20 mL/kg,天马颗粒低剂量5.39 g/kg;天马颗粒正常剂量10.79 g/kg;灌胃给药,隔天1次,持续2周。摘除肿瘤组织,测量肿瘤体重及体积,免疫组化检测TLR4、NF-κB、Klotho表达量。结果:同对照组相比,天马颗粒正常剂量组肿瘤组织中TLR4、NF-κB表达降低,Klotho表达明显增加(P<0.05);天马颗粒正常剂量组与天马颗粒低剂量组相比,肿瘤组织中TLR4、NF-κB表达未见明显差异(P>0.05),Klotho表达升高(P<0.05)。结论:天马颗粒剂可抑制TLR4/NF-κB通路活性,促进Klotho表达,降低肿瘤中炎性因子表达,改善肿瘤炎症微环境。Objective:To observe the effect of Tianma granule on the expression of TLR4,NF-κB and Klotho in nude mice with colorectal cancer.Methods:SPF nude mice were used as the research object,and HCT116 cell line was subcutaneously planted to construct a nude mouse xenograft model.The nude mice were randomly divided into three groups:control group,Tianma granuLe low-dose group and Tianma granule normal-dose group.The control group was given normal saline 20mL/kg,Tianma granuLe low-dose 5.39g/kg;tianma granuLe normal dose 10.79 g/kg;intragastric administration,once every other day for 2 weeks.The tumor tissues were removed,and the tumor weight and volume were measured.The expression of TLR4,NF-κB and Klotho was detected by immunohistochemistry.Results:Compared with the control group,the expression of TLR4 and NF-κB in the tumor tissue of the normal dose group of Tianma granuLes decreased,the expression of Klotho increased significantly(P<0.05),and the expression of Klotho increased(P<0.05).Conclusion:Tianma granuLes can inhibit the activity of TLR4/NF-κB pathway,promote the expression of Klotho,reduce the expression of inflammatory factors in tumors,and improve the inflammatory microenvironment of tumors.

关 键 词：天马颗粒剂 大肠癌 TLR4 NF-ΚB KLOTHO 

分 类 号：R285.5[医药卫生—中药学]