便血方剂“核心配伍-作用靶点”的关联性研究  

Study on the Correlation of“Core Compatibility Action Target”of Hematochezia Prescription

作　　者：张媛婷 胡宗仁 梁璠琦 别明珂 欧广洋 陈新宇[1] Zhang Yuanting;Hu Zongren;Liang Fanqi;Bie Mingke;Ou Guangyang;Chen Xinyu(The First Hospital Affiliated to Hunan University of Chinese Medicine,Changsha 410021,China;Graduate School,Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Medical College,Huaihua 418000,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410021 [2]湖南中医药大学研究生院,湖南长沙410208 [3]湖南医药学院,湖南怀化418000

出　　处：《亚太传统医药》2024年第2期136-141,共6页Asia-Pacific Traditional Medicine

基　　金：全国名老中医药专家陈新宇传承工作室建设项目(国中医药人教函[2022]75号);国家自然科学基金资助项目(81704061,81173213);湖南省科学技术厅重点领域研发计划(2019SK2321);湖南省发改委创新引导专项(湘发改投资2019-412号);“四时调阳”治未病湖南省工程研究中心(湘发改高技[2020]1006号)。

摘　　要：目的:研究治疗便血方剂的用药规律及其“核心配伍-作用靶点”的关联性。方法:从《中医方剂大辞典》中筛选治疗便血的方剂,录入数据库,进行频数、聚类和关联分析,从中发现配伍组方规律。找到核心配伍组合后,再使用中药分子机制的生物信息学分析工具(Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine,BATMAN-TCM)对核心配伍组合进行活性成分-靶点预测,从而分析核心配伍组合的共同作用靶点。结果:共收录便血方剂616首,涉及中药340味;排名前10的高频药物依次是当归、甘草、黄连、芍药、枳壳、生地黄、黄芩、地榆、槐花、黄芪;主要使用的中药类别依次是补虚药、清热药、止血药。发现生地黄、黄芩、赤芍,人参、白术、茯苓,侧柏叶、地榆、槐花,当归和川芎等是治疗便血的四组核心配伍组合;经分析发现这四组核心配伍组合均有AR、ESR1、NR3C1、PGR、VDR这5个共同作用靶点,即治疗便血的11个核心药物有5个共同作用靶点。结论:《中医方剂大辞典》所载便血方剂的核心配伍组合关联的AR、NR3C1、PGR、VDR等靶点与肠炎、结直肠癌、胃癌等常导致便血的相关疾病密切相关,提示其“核心配伍-作用靶点”的关联性对于指导临床用药以及相关基础研究和新药开发有一定参考意义。Objective:To study the rule of drug use and the relationship between“Core Compatibility Action Target”of the formula for treating blood in stool.Methods:Prescriptions for the treatment of hematochezia were selected from the Dictionary of Traditional Chinese Medicine Prescriptions,and entered into the database for frequency,clustering and correlation analysis,so as to find the rule of compatibility.After the core compatibility combination is found,the bioinformatics analysis tool of the molecular mechanism of traditional Chinese medicine is used to predict the active ingredient target of the core compatibility combination,so as to analyze the common action target of the core compatibility combination.Results:616 pieces of blood stool prescriptions were collected,involving 340 Chinese herbs;The top ten high-frequency drugs were Angelica sinensis,Licorice,Coptis chinensis,Peony,Trifoliate orange,Rehmannia root,Scutellaria baicalensis,Sanguisorba officinalis,Sophora japonica,and Astragalus membranaceus;The main categories of traditional Chinese medicine used were tonic,antipyretic and hemostatic;The raw Rehmannia glutinosa,Scutellaria baicalensis and Paeonia rubra were found;Ginseng,Atractylodes macrocephala,Poria cocos;Platycladus orientalis leaves,Sanguisorba officinalis and Sophora japonica flowers;Angelica sinensis and Ligusticum chuanxiong are the four core compatibility combinations for the treatment of hematochezia;Through analysis,it is found that the four core compatibility combinations have five common action targets,namely AR,ESR1,NR3C1,PGR and VDR,that is,the 11 core drugs for the treatment of hematochezia have five common action targets.Conclusion:AR,NR3C1,PGR,VDR and other targets related to the core compatibility combination of blood stool formulas in the Dictionary of Traditional Chinese Medicine Prescriptions are closely related to the related diseases that often cause blood stool,such as enteritis,colorectal cancer,gastric cancer,etc.,suggesting that the correlation of its“Core Compatibility A

关 键 词：中医方剂大辞典 便血 方剂 配伍规律 核心药物 作用靶点 

分 类 号：R289[医药卫生—方剂学]