全自动生化分析仪锑铈催化分光光度法测定水碘的方法研究  

A method of antimony cerium catalytic spectrophotometric determination of water iodine using a fully automatic biochemical analyzer

作　　者：徐署东 纵瑞涵 姜健 王开倩 谢继安[1] 丁刚[2] Xu Shudong;Zong Ruihan;Jiang Jian;Wang Kaiqian;Xie Ji'an;Ding Gang(Department of Physical and Chemical Laboratory,Anhui Center for Disease Control and Prevention,Hefei 230601,China;Office of Anhui Center for Disease Control and Prevention,Hefei 230601,China)

机构地区：[1]安徽省疾病预防控制中心理化检验室,合肥230601 [2]安徽省疾病预防控制中心办公室,合肥230601

出　　处：《中华地方病学杂志》2023年第12期1004-1007,共4页Chinese Journal of Endemiology

基　　金：中国疾控中心公共卫生领域卫生健康标准化前期研究项目(BZ2023-Q003)。

摘　　要：目的探讨全自动生化分析仪锑铈催化分光光度法(以下简称本方法)测定水碘的应用。方法利用碘催化锑铈氧化还原反应的原理,在0~100μg/L碘质量浓度范围内测定水碘含量,从本方法的检出限、精密度、准确度(水中碘成分分析标准物质GBW09113j和GBW09114j测定及加标回收实验)等方面进行效果验证;并与国家碘缺乏病参照实验室推荐的砷铈催化分光光度法进行方法比对实验。结果在0~100μg/L碘质量浓度范围内,本方法的定性和定量检出限分别为0.81和2.70μg/L(取样量为35μl)。精密度实验中,不同碘质量浓度水样的相对标准偏差范围为1.2%~4.0%。水中碘成分分析标准物质GBW09113j、GBW09114j测定结果均在给定的标准值范围内;低、中、高碘质量浓度水样的加标回收率范围为101.0%~106.0%,总平均加标回收率为103.2%。方法比对实验结果显示,两种方法水碘测定结果比较差异无统计学意义(t=-0.78,P=0.779)。结论本方法的检出限低、精密度高、准确性好,适合碘缺乏病病情监测中大批量样品的检测。Objective To study the application of antimony cerium catalytic spectrophotometry using a fully automatic biochemical analyzer(hereinafter referred to as this method)in the determination of water iodine.Methods Based on the principle of antimony cerium reoxidation reduction reaction catalyzed by iodine,the iodine content in water was determined in the range of 0-100μg/L iodine mass concentration.The detection limit,precision and accuracy(determination of standard substances GBW09113j and GBW09114j for iodine composition analysis in water and the experiment of standard recovery)of this method were verified.This method was compared with the arsenic and cerium catalytic spectrophotometry recommended by the National Reference Laboratory for Iodine Deficiency Disorders.Results Within the range of 0-100μg/L iodine mass concentration,the qualitative and quantitative detection limits of this method were 0.81 and 2.70μg/L,respectively(sampling quantity was 35μl).In the precision experiment,the relative standard deviation of water samples with different iodine mass concentrations ranged from 1.2%to 4.0%.The determination results of the standard substances GBW09113j and GBW09114j for iodine composition analysis in water were both within the given standard value range.The standard recovery rates of water samples with low,medium and high iodine mass concentrations ranged from 101.0%to 106.0%,and the total average standard recovery rate was 103.2%.The results of the method comparison experiment showed that there was no statistically significant difference in the results of water iodine determination between the two methods(t=-0.78,P=0.779).Conclusion This method has a low detection limit,high precision and good accuracy,making it suitable for the detection of large quantities of samples in the monitoring of iodine deficiency disorders.

关 键 词：碘 饮水 全自动生化分析仪 锑铈催化分光光度法 

分 类 号：R123.1[医药卫生—环境卫生学]