更欣汤对围绝经期失眠模型大鼠血清雌激素水平及下丘脑雌激素受体的影响  

Effects of Gengxin Tang on serum estrogen levels and hypothalamic estrogen receptors in a perimenopausal insomnia model in rat

作　　者：王艺璇[1] 汤玲[2] 孙天琳 史梅莹[4] 陆义芹[2] 李修恒 WANG Yixuan;TANG Ling;SUN Tianlin;SHI Meiying;LU Yiqin;LI Xiuheng(Chengdu University of Traditional Chinese Medicine,Chengdu 610032,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Beijing First Hospital of Integrated Traditional Chinese and Western Medicine,Beijing 100000,China;Wangjing Hospital,China Academy of Chinese Medical Sciences,Beijing 100102,China)

机构地区：[1]成都中医药大学,成都610032 [2]北京中医药大学东直门医院,北京100700 [3]北京市第一中西医结合医院,北京100000 [4]中国中医科学院望京医院,北京100102

出　　处：《中华中医药杂志》2024年第1期147-151,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：第四届国医大师传承工作室建设项目(No.401091401);中国民族医药学会科研项目(No.2021Z1225-340102);名医传承、薪火“3+3工程”(No.JX336)。

摘　　要：目的:探讨国医大师肖承悰教授临床验方“更欣汤”对围绝经期失眠模型大鼠行为、血清雌激素水平及下丘脑雌激素受体表达的影响。方法:选取60只7周龄雌性SD大鼠,采用雷公藤多苷溶液灌胃联合对氯苯丙氨酸(PCPA)混悬液腹腔注射诱导围绝经期失眠大鼠模型。将26只造模成功的大鼠随机分为模型组和中药组各13只,另设空白组12只;中药组每日予更欣汤颗粒剂溶液1.8 g/kg灌胃,空白组和模型组大鼠每日予等量0.9%氯化钠溶液灌胃,周期14 d。利用旷场实验(OFT)观察大鼠行为学改变,放射免疫分析法检测大鼠血清雌二醇(E2)水平,Western Blot法检测大鼠下丘脑雌激素受体(ER)蛋白表达,RT-qPCR法检测大鼠下丘脑ERα、βmRNA表达。结果:与空白组比较,模型组大鼠OFT水平与垂直活动得分均显著降低(P<0.05),修饰次数显著增多(P<0.01),血清E2显著下降(P<0.01),下丘脑ERα、ERβ蛋白表达显著下降(P<0.05),ERα/ERβ比值显著升高(P<0.01);与模型组比较,中药组大鼠修饰次数显著下降(P<0.01),下丘脑ERβ蛋白表达显著升高(P<0.05),ERα/ERβ比值下降(P<0.01)。结论:更欣汤能够上调围绝经期失眠模型大鼠下丘脑ER不同亚型蛋白表达,从而改善大鼠睡眠状态。Objective:To explore the effects of professor XIAO Chengjun,a renowned Chinese medicine practitioner,on the behavior,serum estrogen levels,and hypothalamic estrogen receptor expression of perimenopausal insomnia model rats using the clinical formula‘Gengxin Tang’.Methods:Sixty 7-week-old female SD rats were selected,and a perimenopausal insomnia rat model was induced by gavage of Tripterygium wilfordii glycoside solution combined with intraperitoneal injection of p-chlorophenylalanine(PCPA)suspension.Twenty-six successfully modeled rats were randomly divided into a model group and a traditional Chinese medicine group,with 13 rats in each group,and an additional 12 rats in a blank group;The traditional Chinese medicine group was orally administered 1.8 g/kg of Gengxin Tang granules solution daily,while the blank group and model group rats were orally administered an equal amount of 0.9%sodium chloride solution daily for 14 days.Observing behavioral changes in rats using open field experiments(OFT),detecting serum estradiol(E2)levels using radioimmunoassay,detecting estrogen receptor(ER)protein expression in the hypothalamus of rats using Western Blot,and detecting ER in the hypothalamus of rats using RT-qPCRα,βmRNA expression.Results:Compared with the blank group,the OFT level and vertical activity score of the model group rats were significantly reduced(P<0.05),the number of modifications was significantly increased(P<0.01),serum E2 was significantly decreased(P<0.01),and hypothalamic ER was significantly reducedα,ERβsignificant decrease in protein expression(P<0.05),ERα/ERβratio significantly increased(P<0.01);Compared with the model group,the modification frequency of the Chinese medicine group rats was significantly decreased(P<0.01),and the hypothalamic ER decreasedβsignificant increase in protein expression(P<0.05),ERα/ERβratio decreased(P<0.01).Conclusion:Gengxin Tang can upregulate the serum E2 level and the expression of different subtypes of ER proteins and genes in the hypothalamus of perim

关 键 词：更欣汤 围绝经期 失眠 矿场实验 下丘脑 雌激素 雌激素受体 

分 类 号：R285.5[医药卫生—中药学]