深浅不同针刺对慢性束缚应激大鼠抑郁样行为及皮质神经营养因子的影响  

Effects of deep and superficial needling on depression-like behavior and cortical neurotrophic factors in rats after chronic immobilization stress

作　　者：刘群 周晨 张雯晰 赵玉雪[1] 辛娟娟[1] 高俊虹[1] 喻晓春[1] LIU Qun;ZHOU Chen;ZHANG Wenxi;ZHAO Yuxue;XIN Juanjuan;GAO Junhong;YU Xiaochun(Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院针灸研究所,北京100700

出　　处：《中华中医药杂志》2024年第1期152-156,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：中央级公益性科研院所基本科研业务费专项资金(No.ZZ202017007)。

摘　　要：目的:观察深浅不同针刺用于慢性束缚应激抑郁大鼠的抗抑郁效应及对皮质神经营养因子的影响。方法:24只SD大鼠随机分为对照组、模型组、深刺组及浅刺组,每组6只。除对照组外,均采用21 d慢性束缚应激的方法建立抑郁模型,深刺组和浅刺组分别给予“太冲”穴直刺和“太冲”穴平刺治疗,1次/d,持续21 d。通过大鼠第21天体质量、旷场实验和新环境抑制进食实验来评价抑郁造模和治疗效果。采用荧光定量PCR法及Western Blot法检测大鼠皮质神经生长因子(NGF)、脑源性神经营养因子(BDNF)的mRNA及蛋白的表达情况。结果:模型组大鼠体质量显著低于对照组(P<0.01),与模型组比较,深刺组体质量显著增加(P<0.05)。模型组大鼠5 min总运动距离、边缘区运动距离、中央区运动距离、总平均速度、边缘区平均速度和中央区平均速度均显著低于对照组(P<0.01);深刺组总运动距离、边缘区运动距离、总平均速度、边缘区平均速度显著高于模型组(P<0.05)。模型组进食潜伏期显著高于对照组(P<0.01);与模型组比较,深刺组和浅刺组进食潜伏期均显著缩短(P<0.01),深刺组较浅刺组潜伏期更短(P<0.01)。与对照组比较,模型组大鼠皮质中NGF、BDNF mRNA和蛋白表达水平均显著下降(P<0.05,P<0.01);与模型组大鼠比较,深刺组皮质NGF、BDNF mRNA和蛋白的表达水平显著升高(P<0.05)。结论:深刺“太冲”穴能显著改善慢性束缚应激大鼠抑郁样行为,优于浅刺治疗,其作用机制可能与改善皮质NGF和BDNF异常表达有关。Objective:To observe the anti-depressant effect of deep and superficial needling on chronic restraint stress induced depressive rats,and to explore the influence of acupuncture on expression of neurotrophic factors in cortex.Methods:A total of 24 SD rats were randomly divided into control group,model group,deep needling group and superficial needling group,with 6 rats in each group.Except for the control group,the depression model was established by 21-day chronic immobilization stress.The deep needling group and superficial needling group were given perpendicular needling and horizontal needling at‘Taichong’(LR 3)point respectively,once a day for 21 days.The modeling and treatment effects of depression were evaluated using bodyweight on the 21st day,open field test,and novelty suppressed feeding test.Fluorescence quantitative PCR and Western Blot technique were applied for detecting gene and protein expression of nerve growth factor(NGF)and brain-derived neurotrophic factor(BDNF)in cortex respestively.Results:Compared with the control group,the body weight of the model group was significantly lower(P<0.01);and the body weight of the deep needling group was higher than that of the model group(P<0.05).In the model group,the total movement distance in 5 minutes,the movement distance in the side zone,the movement distance in the central zone,the total average velocity,the average velocity in the side zone and the average velocity in the central zone were all significantly lower than those of the control group(P<0.01).The total movement distance,the movement distance in the side zone,the total average velocity and the average velocity in the side zone of the deep needling group were significantly higher than those in the model group(P<0.05).The latency of feeding of the model group was significantly higher than that of the control group(P<0.01);Compared with the model group,the latency of feeding was shorter in both the deep needling group and the superficial needling group(P<0.01),and the latency of the deep nee

关 键 词：深浅针刺 慢性束缚应激 抗抑郁效应 皮质 神经生长因子 脑源性神经营养因子 

分 类 号：R245[医药卫生—针灸推拿学]