基于16S rRNA测序探讨穿山龙总皂苷对高尿酸血症大鼠的作用及机制  

Discussion on the effect and mechanism of total saponins of Dioscoreae Nipponicae Rhizoma on hyperuricemia rats based on 16S rRNA sequencing

作　　者：刘树民[1] 卢意 轩秀秀 张楠 修海南 王天雨 于栋华[1] LIU Shumin;LU Yi;XUAN Xiuxiu;ZHANG Nan;XIU Hainan;WANG Tianyu;YU Donghua(Institute of Traditional Chinese Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040

出　　处：《中华中医药杂志》2024年第1期163-167,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：黑龙江省自然科学基金项目(No.ZD2020H006);国家自然科学基金面上项目(No.82074149);国家自然科学基金青年科学基金项目(No.81803871)。

摘　　要：目的:探讨穿山龙总皂苷对高尿酸血症大鼠肠道菌群的影响。方法:将63只大鼠随机分成空白组(n=10)和造模组(n=53),10%酵母饲料饲养和灌胃氧嗪酸钾溶液造模14 d,将成模大鼠随机分成模型组,穿山龙总皂苷低、中、高剂量组及别嘌醇组,各10只。第15天开始给药,持续2周。采用全自动化生化仪分析检测血尿酸、肌酐、尿素氮的含量,进一步采用16S rRNA测序检测肠道菌群变化。结果:各给药组均能显著降低尿酸水平(P<0.01),其中穿山龙总皂苷高剂量组能降低尿素氮水平(P<0.05),调节肠道菌群物种丰度及均匀度,回调变形菌门丰度,减小拟杆菌门丰度,增加乳酸杆菌属、Ruminococcaceae_Ruminococcus丰度,降低拟杆菌属和Parabacteroides属丰度。结论:穿山龙总皂苷能够显著降低高尿酸血症大鼠尿酸水平,推测其作用机制可能与调控肠道菌群紊乱有关。Objective:To investigate the effects of total saponins of Dioscoreae Nipponicae Rhizoma on intestinal flora in hyperuricemia rats.Methods:A total of 63 rats were randomly divided into control group(n=10)and model group(n=53).The model group was fed with 10%yeast feed and intragaxed with potassium oxyazinate solution for 14 days.Then the rats were randomly divided into model group,low-,middle-and high-dose of total saponins of Diascoreae Nipponicae Rhizoma group and allopurinol group,with 10 rats in each group.The rats were treated with allopurinol and total saponins of Dioscoreae Nipponicae Rhizoma from the 15th day and lasted for 2 weeks.The contents of serum uric acid,creatinine and urea nitrogen were analyzed by fully automated biochemical instrument,and the changes of intestinal flora were further detected by 16S rRNA sequencing.Results:All administration groups can significantly reduce uric acid level(P<0.01),and the high-dose of total saponins of Dioscoreae Nipponicae Rhizoma group can reduce urea nitrogen level(P<0.05),regulate the species abundance and evenness of intestinal flora,adjust the abundance of Proteobacteria,reduce the abundance of Bacteroidetes,and increase the abundance of Lactobacillus and Ruminococcaceae_Ruminococcus.Meanwhile,the abundance of Bacteroides and Parabacteroides was decreased.Conclusion:The total saponins of Dioscoreae Nipponicae Rhizoma can significantly reduce the level of uric acid in hyperuricemia rats,suggesting that the mechanism may be related to the regulation of intestinal flora disorder.

关 键 词：穿山龙总皂苷 高尿酸血症 肠道菌群 16S rRNA 尿酸 

分 类 号：R285.5[医药卫生—中药学]