通络开痹片的消炎镇痛作用及机制  

Anti-inflammatory and analgesic effects and mechanism of Tongluo Kaibi Tablet

作　　者：卫博文 刘维[1,2] 高晶月 王爱华 刘春柳 丁久力 郭小静 王怡文 WEI Bowen;LIU Wei;GAO Jingyue;WANG Aihua;LIU Chunliu;DING Jiuli;GUO Xiaojing;WANG Yiwen(First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;National ClinicalResearch Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300193,China)

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]国家中医针灸临床医学研究中心,天津300193

出　　处：《中华中医药杂志》2024年第1期387-392,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家中医药管理局名老中医传承工作室(No.975022)。

摘　　要：目的:探究通络开痹片对甲醛诱导的大鼠疼痛模型的消炎镇痛作用及机制。方法:雄性SD大鼠24只随机分为空白组、模型组、马钱子组及通络开痹片组,各6只。通过足跖皮下注射5%甲醛构建大鼠疼痛模型。记录注射后第一时相(0—10 min)及第二时相(15—30 min)大鼠疼痛反应次数,6、24及48 h大鼠足跖肿胀度,HE染色观察足跖组织病理变化,ELISA检测大鼠血清中白细胞介素-1β(IL-1β)、前列腺素E_(2)(PGE_(2))、白细胞介素-6(IL-6)、白细胞介素-18(IL-18)及肿瘤坏死因子-α(TNF-α)水平,Western Blot检测足跖组织中NOD样受体蛋白3(NLRP3)及环氧合酶-2(COX-2)蛋白表达水平,qRT-PCR检测足跖组织中NLRP3、前列腺素G/H合成酶2(PTGS2)、IL-1β、IL-6及TNF-αmRNA水平。结果:与空白组比较,模型组大鼠疼痛反应次数及足跖肿胀度显著增加(P<0.01),足跖组织肌细胞形态改变,排列紊乱,组织界限模糊,大量炎症细胞浸润,血清IL-1β、PGE_(2)、IL-6、IL-18及TNF-α水平,足跖组织中NLRP3、COX-2蛋白水平及NLRP3、PTGS2、IL-1β、IL-6、TNF-αmRNA水平均显著升高(P<0.01);与模型组比较,马钱子组第一时相内疼痛反应次数显著减少(P<0.01),足跖肿胀度在6、48 h也显著减轻(P<0.01),足跖组织肌细胞形态改变,组织界限相对清楚,少量炎症细胞浸润,PTGS2、IL-6的mRNA表达水平均显著降低(P<0.01),NLRP3、IL-1β、PGE_(2)、IL-6、IL-18及TNF-α水平均显著降低(P<0.05,P<0.01);通络开痹片组第一时相内疼痛反应次数显著减少(P<0.01),足跖肿胀度在6、48 h显著减轻(P<0.01),肌细胞排列整齐,组织界限较清楚,散在炎症细胞分布,NLRP3、IL-1β、COX-2、PGE_(2)、IL-6、IL-18及TNF-α的mRNA及蛋白表达水平均显著降低(P<0.05,P<0.01)。与马钱子组比较,通络开痹片组炎性浸润程度显著减轻,NLRP3、IL-1β、COX-2、PGE_(2)、IL-6、IL-18、TNF-α蛋白及mRNA表达水平显著下降(P<0.05,P<0.01)。结论:通络开痹片可以通过Objective:To investigate the anti-inflammatory and analgesic effects and mechanisms of Tongluo Kaibi Tablet on formaldehyde-induced pain model in rats.Methods:Twenty-four male SD rats were randomly divided into the blank group,model group,Strychni Semen group,and Tongluo Kaibi Tablet group,with 6 rats in each group.The pain model of rat was induced by subcutaneous injection of 5%formaldehyde into the plantar surface.The number of pain responses of the rats in the first(0-10 min)and second(15-30 min)phases after injection was recorded,as well as the plantar swelling at 6,24,and 48 h.The histopathological changes of the plantar were observed by HE staining.The levels of interleukin-1β(IL-1β),prostaglandin E,(PGE_(2)),IL-6,IL-18 and tumor necrosis factor-α(TNF-α)in rat serum were measured by ELISA,nucleotide oligomerization domain-like receptor protein-3(NLRP3)and cyclooxygenase-2(COX-2)in plantar tissues by Western Blot,and NLRP3,prostaglandin G/H synthase 2(PTGS2),IL-1β,IL-6 and TNF-αmRNA in plantar tissues by qRT-PCR.Results:Compared with the blank group,the rats in model group had significantly increased the number of pain responses and the degree of plantar swelling(P<0.01),the myocytes morphology in plantar tissues was changed,the arrangement was disordered,the tissue boundaries were blurred,a large number of inflammatory cells infiltrated,and serum IL-1β,PGE_(2),IL-6,IL-18,and TNF-α levels,NLRP3,COX-2 protein levels,as well as NLRP3,PTGS2,IL-1β,IL-6,TNF-αmRNA levels in plantar tssue were significantly increased(P<0.01);compared with the model group,the number of pain responses within the first phase was significantly reduced in the Strychni Semen group(P<0.01),and the plantar swelling at 6 and 48 h was also significantly reduced(P<0.01),the myocyte morphology of the plantar tissue was changed,the tissue boundaries were relatively clear,a few of inflammatory cells were infiltrated,and the mRNA expression levels of PTGS2 and IL-6 were significantly decreased(P<0.01),NLRP3,IL-1β,PGE_(2),IL-6,IL-18 and

关 键 词：通络开痹片 马钱子 疼痛 NLRP3炎症小体 消炎镇痛 

分 类 号：R285.5[医药卫生—中药学]