海南省东方市甜瓜细菌性果斑病病原鉴定  

Identification of Pathogen Causing Bacterial Fruit Blotch of Melon in Hainan

作　　者：李鹏声 黄清泰 范咏梅 王萌[1,2] 杨叶 LI Pengsheng;HUANG Qingtai;FAN Yongmei;WANG Meng;YANG Ye(Sanya Institute of Breeding and Multiplication,Hainan University,Hainan Sanya 572025,China;School of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学三亚南繁研究院,海南三亚572025 [2]海南大学热带农林学院,海口570228

出　　处：《中国农业科技导报》2024年第3期117-123,共7页Journal of Agricultural Science and Technology

基　　金：海南省自然科学基金项目(321RC457);海南省东方市农业绿色发展先行先试支撑体系建设项目(20210714)。

摘　　要：细菌性果斑病严重影响甜瓜生产,为确定其病原菌种类,从海南省东方市种植基地采集大量疑似细菌性果斑病的甜瓜样本,进行病原菌分离纯化,并按柯赫法则对获得的菌株进行致病性测定。通过菌落形态观察、生理生化测定及16S rDNA序列比对分析,对分离病原菌进行鉴定。结果表明,大田采集的所有甜瓜病果中均分离到培养性状一致的细菌,细菌分离率为100%;所有分离菌株均对甜瓜果实具有致病性,该病原菌革兰氏反应呈阴性,其菌落形态及生理生化测定结果与已报道的西瓜嗜酸菌(Acidovorax citrulli)一致;BLAST比对与A. citrulli菌株的同源性可达100%,系统发育分析也进一步证实该病菌为A. citrulli。研究结果确定了甜瓜果斑病的致病菌,为该病的有效防治提供了依据。To identify the pathogen which seriously caused fruit blotch disease in melo,the melon fruit samples suspected bacterial fruit blotch disease were collected from Dongfang city,Hainan province.Pathogen was isolated and purified from samples,and the pathogenicity of bacterium strains was confirmed according to Koch’s postulate.The identification of the pathogenic strains was carried out according to the colony morphology,physiological and biochemical analysis and 16S rDNA sequences analysis.The results showed that the isolated bacterium with similar cultural characteristics was obtained from all diseased samples,which the separation rate of bacteria was 100%.All of tested strains showed pathogenic to melon fruits.Strains were identified as Gram-negative bacterium,which colony morphology,physiology and biochemistry shared similarly with that of Acidovorax citrull.BLAST analysis showed that the 16S rDNA sequences had 100%homology with that of A.citrulli,and the phylogenetic analysis showed the pathogen was A.citrulli.Above results determined the pathogen of melon fruit blotch,which provided a basis for effective prevention and control of this disease.

关 键 词：甜瓜 细菌性果斑病 病原菌鉴定 西瓜嗜酸菌 海南省东方市 

分 类 号：S652[农业科学—果树学] S436.5[农业科学—园艺学]