PoWRKY71 is involved in Paeonia ostii resistance to drought stress by directly regulating light-harvesting chlorophyll a/b-binding 151 gene  

作　　者：Yuting Luan Zijie Chen Ziwen Fang Xingqi Huang Daqiu Zhao Jun Tao 

机构地区：[1]College of Horticulture and Landscape Architecture,Yangzhou University,Yangzhou 225009,China [2]Department of Biochemistry,Purdue University,West Lafayette,IN 47907,USA [3]Joint International Research Laboratory of Agriculture and Agri-Product Safety,the Ministry of Education of China,Yangzhou University,Yangzhou 225009,China

出　　处：《Horticulture Research》2023年第11期1-12,共12页园艺研究（英文）

基　　金：supported by National Forest and Grass Science and Technology Innovation and Development Research Project(2023132012);Modern Agriculture(Flower)Industrial Technology System of Jiangsu Province(JATS[2023]489);Jiangsu Agricultural Science and Technology Innovation Foundation of China[CX(22)3186];Science and Technology Special Project of North Jiangsu Province[SZ-SQ2021041];Forestry Science and Technology Promotion Project of Jiangsu Province[LYKJ[2021]28];Qing Lan Project of Jiangsu Province and High-Level Talent Support Program of Yangzhou University.

摘　　要：Although the functions of WRKY transcription factors in drought resistance are well known,their regulatory mechanisms in response to drought by stabilising photosynthesis remain unclear.Here,a differentially expressed PoWRKY71 gene that was highly expressed in drought-treated Paeonia ostii leaves was identified through transcriptome analysis.PoWRKY71 positively responded to drought stress with significantly enhanced expression patterns and overexpressing PoWRKY71 in tobacco greatly improved plant tolerance to drought stress,whereas silencing PoWRKY71 in P.ostii resulted in a drought-intolerant phenotype.Furthermore,lower chlorophyll contents,photosynthesis,and inhibited expression of photosynthesis-related light-harvesting chlorophyll a/b-binding 151(CAB151)gene were found in PoWRKY71-silenced P.ostii.Meanwhile,a homologous system indicated that drought treatment increased PoCAB151 promoter activity.Interactive assays revealed that PoWRKY71 directly bound on the W-box element of PoCAB151 promoter and activated its transcription.In addition,PoCAB151 overexpressing plants demonstrated increased drought tolerance,together with significantly higher chlorophyll contents and photosynthesis,whereas these indices were dramatically lower in PoCAB151-silenced P.ostii.The above results indicated that PoWRKY71 activated the expression of PoCAB151,thus stabilising photosynthesis via regulating chloroplast homeostasis and chlorophyll content in P.ostii under drought stress.This study reveals a novel drought-resistancemechanism in plants and provides a feasible strategy for improving plant drought resistance via stabilising photosynthesis.

关 键 词：HARVESTING DROUGHT RESISTANCE 

分 类 号：S685.11[农业科学—观赏园艺]