A chromosome-level genome assembly provides insights into Cornus wilsoniana evolution, oil biosynthesis, and floral bud development  

作　　者：Zhenxiang He Haoyu Chao Xinkai Zhou Qingyang Ni Yueming Hu Ranran Yu Minghuai Wang Changzhu Li Jingzhen Chen Yunzhu Chen Yong Chen Chunyi Cui Liangbo Zhang Ming Chen Dijun Chen 

机构地区：[1]State Key Laboratory of Pharmaceutical Biotechnology,School of Life Sciences,Nanjing University,Nanjing 210023,China [2]Department of Bioinformatics,College of Life Sciences,Zhejiang University,Hangzhou 310058,China [3]Forest Protection Department,Guangdong Academy of Forestry,Guangzhou 510520,China [4]State Key Laboratory of Utilization of Woody Oil Resource,Hunan Academy of Forestry,Changsha 410004,China [5]Xishan Forest Farm,Dazu District,Chongqing 402360,China [6]Longshan Forest Farm,Lechang 512221,China [7]Hunan Horticultural Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China

出　　处：《Horticulture Research》2023年第11期27-44,共18页园艺研究（英文）

基　　金：supported by the National Natural Science Foundation of China(31770767,32070656,32070677 and 32270709);Foundation of State Key Laboratory of Utilization of Woody Oil Resource(GZKF202201);Hunan Province Key Research and Development Program Project(2018NK2044);Jiangsu Collaborative Innovation Center for Modern Crop Production and Collaborative Innovation Center for Modern Crop Production cosponsored by province and ministry.

摘　　要：Cornus wilsoniana W.is a woody oil plant with high oil content and strong hypolipidemic effects,making it a valuable species for medicinal,landscaping,and ecological purposes in China.To advance genetic research on this species,we employed PacBio together with Hi-C data to create a draft genome assembly for C.wilsoniana.Based on an 11-chromosome anchored chromosome-level assembly,the estimated genome size was determined to be 843.51 Mb.The N50 contig size and N50 scaffold size were calculated to be 4.49 and 78.00 Mb,respectively.Furthermore,30474 protein-coding genes were annotated.Comparative genomics analysis revealed that C.wilsoniana diverged from its closest species∼12.46 million years ago(Mya).Furthermore,the divergence between Cornaceae and Nyssaceae occurred>62.22 Mya.We also found evidence of whole-genome duplication events and whole-genome triplicationγ,occurring at∼44.90 and 115.86 Mya.We further inferred the origins of chromosomes,which sheds light on the complex evolutionary history of the karyotype of C.wilsoniana.Through transcriptional and metabolic analysis,we identified two FAD2 homologous genes that may play a crucial role in controlling the oleic to linoleic acid ratio.We further investigated the correlation between metabolites and genes and identified 33 MADS-TF homologous genes that may affect f lower morphology in C.wilsoniana.Overall,this study lays the groundwork for future research aimed at identifying the genetic basis of crucial traits in C.wilsoniana.

关 键 词：assembly EVOLUTION FLORAL 

分 类 号：S567[农业科学—中草药栽培]