The telomere-to-telomere gap-free reference genome of wild blueberry (Vaccinium duclouxii) provides its high soluble sugar and anthocyanin accumulation  

作　　者：Tuo Zeng Zhijiao He Jiefang He Wei Lv Shixiang Huang Jiawen Li Liyong Zhu Shuang Wan Wanfei Zhou Zhengsong Yang Yatao Zhang Chong Luo Jiawei He Caiyun Wang Liangsheng Wang 

机构地区：[1]School of Life Sciences,Guizhou Normal University,Guiyang 550000,China [2]Institute of Alpine Economic Plant,Yunnan Academy of Agricultural Sciences,Lijiang 674199,Yunnan,China [3]School of Advanced Agricultural Sciences,Peking University,100871 Beijing,China [4]National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,College of Horticulture&Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China [5]Wuhan Benagen Technology Co.,Ltd,Wuhan 430070,China [6]Key Laboratory of Plant Resources,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China [7]China National Botanical Garden,Beijing 100093,China [8]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Horticulture Research》2023年第11期141-154,共14页园艺研究（英文）

基　　金：supported by the National Natural Science Foundation of China(32160695,32160718);the Natural Science Foundation of Guizhou Province[ZK(2022)301],Guizhou Normal University QSXM[2022]19,the Project of Basic Research Plan in Yunnan(202101BC070003;202302AE090005);the Natural Science Research Project of Guizhou Provincial Department of Education[KY(2022)170],the Guizhou Scholarship for Overseas Students[(2019)12], the Innovation Program of Postgraduate Education of Guizhou Province[Qian Jiao He YJSCXJH(2020)098].

摘　　要：Vaccinium duclouxii,endemic to southwestern China,is a berry-producing shrub or small tree belonging to the Ericaceae family,with high nutritive,medicinal,and ornamental value,abundant germplasm resources,and good edible properties.In addition,V.duclouxii exhibits strong tolerance to adverse environmental conditions,making it a promising candidate for research and offering wide-ranging possibilities for utilization.However,the lack of V.duclouxii genome sequence has hampered its development and utilization.Here,a high-quality telomere-to-telomere genome sequence of V.duclouxii was de novo assembled and annotated.All of 12 chromosomes were assembled into gap-free single contigs,providing the highest integrity and quality assembly reported so far for blueberry.The V.duclouxii genome is 573.67 Mb,which encodes 41953 protein-coding genes.Combining transcriptomics and metabolomics analyses,we have uncovered the molecular mechanisms involved in sugar and acid accumulation and anthocyanin biosynthesis in V.duclouxii.This provides essentialmolecular information for further research on the quality of V.duclouxii.Moreover,the high-quality telomere-to-telomere assembly of the V.duclouxii genome will provide insights into the genomic evolution of Vaccinium and support advancements in blueberry genetics and molecular breeding.

关 键 词：SUGAR offering BERRY 

分 类 号：S663[农业科学—果树学]