Genome resequencing reveals the evolutionary history of garlic reproduction traits  

作　　者：Haiping Wang Einat Shemesh-Mayer Jiangjiang Zhang Song Gao Zheng Zeng Zemao Yang Xueyu Zhang Huixia Jia Yanzhou Wang Jiangping Song Xiaohui Zhang Wenlong Yang Qiaoyun He Amir Sherman Lin Li Rina Kamenetsky Touming Liu 

机构地区：[1]State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing,China [2]Institute of Plant Sciences,Agricultural Research Organization-The Volcani Institute,Rishon LeZion,Israel [3]Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,China [4]College of Horticulture and Landscape Architecture,Yangzhou University,Yangzhou,China [5]Industrial Research Institute of garlic(IBFC-Jinxiang),Jinxiang,China [6]College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,China

出　　处：《Horticulture Research》2023年第11期208-221,共14页园艺研究（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(31872946,32172566 to H.W.,32372689 to T.L.);the Scientific Research Foundation of Yangzhou University(5018/137012867 to T.L.);the Shandong Provincial Key Research and Development Program(2023CXPT045 to T.L.);the China Agriculture Research System of MOF and MARA(CARS-24-01 to H.W.).

摘　　要：The propagation of cultivated garlic relies on vegetative cloves,thus flowers become non-essential for reproduction in this species,driving the evolution of reproductive feature-derived traits.To obtain insights into the evolutionary alteration of reproductive traits in the clonally propagated garlic,the evolutionary histories of two main reproduction-related traits,bolting and flower differentiation,were explored by genome analyses using 134 accessions displaying wide diversity in these two traits.Resequencing identified 272.8 million variations in the garlic genome,198.0million of which represent novel variants.Population analysis identified five garlic groups that have evolved into two clades.Gene expression,single-cell transcriptome sequencing,and genome-wide trait association analyses have identified numerous candidates that correlate with reproductive transition and flower development,some of which display distinct selection signatures.Selective forces acting on the B-box zinc finger protein-encoding Asa2G00291.1,the global transcription factor group E protein-encoding Asa5G01527.1,and VERNALIZATION INSENSITIVE 3-like Asa3G03399.1 appear to be representative of the evolution of garlic bolting.Plenty of novel genomic variations and trait-related candidates represent valuable resources for biological studies of garlic.Numerous selective signatures from genes associated with the two chosen reproductive traits provide important insights into the evolutionary history of reproduction in this clonally propagated crop.

关 键 词：TRAITS evolutionary SIGNATURE 

分 类 号：S633.4[农业科学—蔬菜学]