印度梨形孢—紫花苜蓿共生体幼苗对镉胁迫生理特性的响应  

Physiological response of Piriformospora indica-Medicago sativa symbiotic seedlings to Cd stress

作　　者：窦晓慧 许婷婷 董智[1] 先露露 王艺颖 李红丽[1] 杨子晋 DOU Xiaohui;XU Tingting;DONG Zhi;XIAN Lulu;WANG Yiying;LI Hongli;YANG Zijin(College of Forestry,Shandong Agricultural University,Mountain Tai Forest Ecosystem Research Station of State Forestry and Grassland Administration,271018,Tai′an,Shandong,China;Feicheng Administrative Examination and Approval Service Bureau,271600,Feicheng,Shandong,China)

机构地区：[1]山东农业大学林学院泰山森林生态系统定位研究站,山东泰安271018 [2]肥城市行政审批服务局,山东肥城271600

出　　处：《中国水土保持科学》2024年第1期114-121,共8页Science of Soil and Water Conservation

基　　金：内蒙古自治区科技重大专项项目“物理化学(微)生物复合型固沙改土材料精准配施工艺的研发”(2019ZD003-3-1);山东省农业科技资金(林业科技创新)课题“黄泛沙地防风治沙型优良经济林草高效栽培技术研究与示范”(2019LY005-03);欧洲投资银行贷款山东沿海防护林工程项目监测与评价(SCSFP-JC)。

摘　　要：为探明印度梨形孢—紫花苜蓿共生体幼苗在镉(Cd)胁迫下生长、生理指标的变化及其对Cd的积累效应,通过水培实验,研究不同质量浓度镉胁迫下(0、5、10、30、50和100 mg/L)印度梨形孢(Piriformospora indica)对紫花苜蓿(Medicago sativa L)幼苗生理特性以及Cd富集和转移的影响。结果表明:1)Cd对紫花苜蓿生长具有“低促高抑”的效应,并且这种效应不受印度梨形孢的影响。在中高质量浓度Cd胁迫下,接种印度梨形孢帮助紫花苜蓿幼苗提高生物量,减少黄叶数,明显改善其生长状况。2)不同质量浓度Cd胁迫下,接种印度梨形孢紫花苜蓿幼苗抗性生理指标显著提升,如可溶性蛋白、可溶性糖、脯氨酸含量以及抗氧化酶活性,且丙二醛含量显著降低。3)接种印度梨形孢增加紫花苜蓿幼苗的生物富集系数,Cd质量浓度为5 mg/L时,生物富集系数较不接种增加86.36%;接种印度梨形孢还能降低Cd在紫花苜蓿幼苗体内的转移系数,Cd质量浓度为10 mg/L时,转移系数较不接种降低68.7%。研究表明印度梨形孢—紫花苜蓿共生体幼苗表现出很强的Cd耐受能力,在Cd胁迫下生长状况和毒性损伤得到明显改善,同时共生体幼苗对Cd具有更好的吸收能力,并且主要积累在根部,抑制Cd上移。结论可为微生物—植物抗逆性研究及其对重金属Cd水土污染的修复提供理论依据,对进一步加强加速水土保持生态修复具有重要意义。[Background]Cadmium pollution is one of the most serious environmental problems in China due to its strong transferability,resistance to degradation and high toxicity.Phytoremediation can not only absorb and enrich heavy metals,but also reconstruct vegetation,restore landscape,preserve and improve soil and water.The combination of plant and endophytic fungi was used to repair heavy metal contaminated soil in order to promote the function of plant remediation of heavy metal pollution.At present,there are few studies on the responses of Piriformospora indica-Medicago sativa symbiotic seedlings to cadmium,especially the physiological mechanism.The aim of this study is to reveal the physiological characteristics of P.indica-M.sativa symbiotic seedlings in response to cadmium stress.[Methods]M.sativa was used as experimental material to study the response of P.indica-M.sativa symbiotic seedlings to different concentrations of cadmium(0,5,10,30,50 and 100 mg/L)through hydroponic experiments.Then the growth and physiological characteristics,antioxidant activity,and accumulation,enrichment and transfer of heavy metal cadmium of P.indica-M.sativa symbiotic seedlings were analyzed.[Results]Cadmium had a“promotion at low concentration and inhibition at high concentration”effect on M.sativa growth,and this effect was not affected by P.indica.Under the stress of medium and high concentration of cadmium,the inoculation of P.indica helped to increase the biomass of M.sativa seedlings,reduce the number of yellow leaves,and obviously improve their growth condition.2)Under different concentrations of cadmium stress,the physiological indexes of resistance of M.sativa seedlings inoculated with P.indica significantly increased,such as the contents of soluble protein,soluble sugar,proline and antioxidant enzyme activities,and the content of malondialdehyde significantly decreased.3)Inoculation of P.indica increased the bio-enrichment coefficient of M.sativa seedlings,for example,when the concentration of cadmium was 5 mg/L,the bio

关 键 词：CD胁迫 印度梨形孢 紫花苜蓿 抗逆生理 转移系数 生物富集系数 

分 类 号：Q945.78[生物学—植物学] S184[农业科学—农业基础科学]