中外猪种间生长发育相关的差异候选基因筛选  

Genome-wide Identification of Candidate Genes Underlying Growth and Development Disparities between Chinese Indigenous and Western Pig Breeds

作　　者：陶璇[1] 梁艳[1] 杨雪梅[1] 汪勇 杨跃奎[1] 孔繁晶 王言 龚建军[1] 朱康平 卫纪 杨少鹏 吕学斌[1] 顾以韧[1] 何志平[1] TAO Xuan;LIANG Yan;YANG Xuemei;WANG Yong;YANG Yuekui;KONG Fanjing;WANG Yan;GONG Jianjun;ZHU Kangping;WEI Ji;YANG Shaopeng;LV Xuebin;GU Yiren;HE Zhiping(Animal Breeding and Genetics Key Laboratory of Sichuan Province,Sichuan Animal Science Academy,Chengdu 610066,China;Luzhou Agricultural and Rural Bureau,Luzhou 646000,China;Luzhou Modern Agriculture Development Promotion Center,Luzhou 646000,China;Sichuan Dekon Livestock Foodstuff Group Co.,Ltd,Chengdu 610207,China;Daocheng County Agriculture and Rural Bureau,Ganzi 627750,China;Baihe County Agriculture and Rural Bureau,Ankang 725800,China)

机构地区：[1]四川省畜牧科学研究院/动物遗传育种四川省重点实验室,四川成都610066 [2]泸州市农业农村局,四川泸州646000 [3]泸州市现代农业发展促进中心,四川泸州646000 [4]四川德康农牧食品集团股份有限公司,四川成都610207 [5]稻城县农牧农村和科技局,四川甘孜627750 [6]白河县农业农村局,陕西安康725800

出　　处：《中国猪业》2024年第1期39-46,共8页China Swine Industry

基　　金：四川省重大科技专项(2021ZDZX0011);四川省重点研发项目(2023YFN0088,2021YFYZ0009,2022YFN0047);财政部和农业农村部:国家现代农业产业技术体系(CARS-35);现代农业产业技术体系四川创新团队(SCCXTD-2024-08);天府农业大师项目(He zhiping 2022);科技城人才计划(优势特色农业产业人才团队计划2023)。

摘　　要：通过对我国地方猪种与国外猪种比较,筛选与生长发育性状相关的差异候选基因,探索猪种之间生长发育差异的分子机制。分别选择5头内江猪和大约克夏猪进行全基因组重测序,以10 kb滑动窗口和1 kb步长计算群体间遗传分化指数(Fst)平均值,并依据Fst筛选差异SNPs。最终在内江猪与大约克夏猪的比较中分别鉴定出48924个非同义SNP,它们高度影响2490个基因。同时,在乙酰辅酶A乙酰转移酶1(ACAT1)、胰岛素样生长因子受体2(IGF2R)和胰岛素样生长因子2 mRNA结合蛋白3(IGF2BP3)基因中检测到3个非同义SNP,这可能影响乙酰辅酶A向乙酰乙酰辅酶A的转化和胰岛素信号通路的正常功能。本研究结果将为探索决定猪表型特征的遗传差异提供基础信息。The study aimed to explore the molecular mechanisms underlying the growth and developmental differences among these pig breeds.Five Neijiang(NJ)pigs and approximately five Yorkshire(YS)pigs were selected for whole-genome resequencing.Differential single-nucleotide polymorphisms(SNPs)were identified through a 10 kb window sliding in 1 kb increments using the Fst method.Ultimately,a total of 48924 non-synonymous single-nucleotide polymorphism sites(nsSNPs)were discovered between NJ and YS pjgs,significantly impacting 2490 genes.Moreover,three nsSNPs were identified in the genes ACAT1,IGF2R and IGF2BP3,potentially affecting the normal functioning of insulin signaling pathways and the conversion of acetyl-CoA to acetoacetyl-CoA.In conclusion,these findings contributed essential knowledge about genetic variations influencing phenotypic features in pigs.

关 键 词：ACAT1 群体遗传分化指数 基因组重测序 分子育种 猪 SNP 

分 类 号：S828[农业科学—畜牧学]