评价快速碳青霉烯酶灭活试验在肠杆菌科细菌碳青霉烯酶表型检测中的应用价值  

Evaluation of the value of a rapid carbapenemase inactivation method for the phenotypic detection of carbapenemases in Enterobacteriaceae

作　　者：孙文悦 周小梅 卢金英 梁栋伟 SUN Wen-yue;ZHOU Xiao-mei;LU Jin-ying(Department of Laboratory Medicine,Nanhai Hospital,Guangdong Provincial People's Hospital,Foshan 528200,China.)

机构地区：[1]广东省人民医院南海医院检验科,广东佛山528200

出　　处：《医师在线》2024年第2期3-6,共4页Journal of Doctors Online

基　　金：佛山市卫生健康局医学科研课题(20230231)。

摘　　要：目的评价快速碳青霉烯酶灭活试验(rCIM)与改良碳青霉烯灭活试验(mCIM)在肠杆菌科细菌碳青霉烯酶表型检测中的应用价值。方法选择86株耐碳青霉烯肠杆菌科细菌,使用聚合酶链式反应(PCR)检测常见碳青霉烯酶基因,比较rCIM与mCIM法检测碳青霉烯酶表型的性能。结果共检测到61株碳青霉烯酶基因型,检出率为70.9%。mCIM法发现阳性菌株61株,阴性菌株25株,rCIM法发现阳性菌株62株,阴性菌株24株。以PCR结果作为金标准,mCIM法的准确度、灵敏度和特异度分别为100.0%、100.0%和100.0%,rCIM法的准确度、灵敏度和特异度分别为98.8%、100.0%和96.0%。结论rCIM法检测肠杆菌科细菌碳青霉烯酶表型,操作简便,不需过夜培养,不需特殊的仪器或试剂,准确度较高。Objective To evaluate the application value of rapid carbapenemase inactivation method(rCIM)and modified carbapenem inactivation method(mCIM)in the phenotypic detection of carbapenemases in Enterobacteriaceae.Methods 86 strains of carbapenem resistant Enterobacteriaceae were selected.The common carbapenemase genes were detected by Polymerase chain reaction(PCR).The performance of rCIM and mCIM in detecting carbapenemase phenotype was compared.Results A total of 61 carbapenemase genotypes were detected,with a detection rate of 70.9%.The mCIM test found 61 positive strains and 25 negative strains,while the rCIM test found 62 positive strains and 24 negative strains.With PCR results as the gold standard,the coincidence rate,sensitivity and specificity of mCIM test were 100.0%,100.0%and 100.0%,respectively,and those of rCIM test were 98.8%,100.0%and 96.0%,respectively.Conclusion The rCIM assay detects the carbapenemase phenotype of Enterobacteriaceae bacteria,which is easy to operate,doesn’t require overnight culture,doesn’t require special instruments or reagents,and has high accuracy.

关 键 词：肠杆菌科 碳青霉烯酶 碳青霉烯酶灭活试验 

分 类 号：R44[医药卫生—诊断学]