DIP2A对肺腺癌的作用机制和药物敏感度分析  

Mechanism of Action and Drug Sensitivity Analysis of DIP2A in Lung Adenocarcinoma

作　　者：孙诗卓 刘思奇 吕铮 周月 张继旭 芦小单 丁亦男[1] SUN Shizhuo;LIU Siqi;L Zheng;ZHOU Yue;ZHANG Jixu;LU Xiaodan;DING Yinan(College of Life Sciences,Changchun Normal University,Changchun 130032,China;Jilin Provincial People’s Hospital,Changchun 130021,China)

机构地区：[1]长春师范大学生命科学学院,吉林长春130032 [2]吉林省人民医院,吉林长春130021

出　　处：《长春师范大学学报》2024年第2期112-117,共6页Journal of Changchun Normal University

基　　金：吉林省预算内基本建设资金项目“原发性甲状旁腺功能亢进症的精准诊断研发和应用”(2023C041-1);吉林省自然科学基金项目“VEGFA参与调控慢性淋巴细胞性甲状腺炎发生发展的机制研究”(YDZJ202201ZYTS148);吉林省中医药科技项目“银杏双黄酮对肺癌细胞杀伤机制的研究”(2023079);吉林省科技厅项目“肺腺癌组织中蛋白酶激活受体-2的表达意义及对A549细胞周期影响的实验研究”(20210203186SF)。

摘　　要：DIP2A对肺腺癌的作用机制和抗癌药物敏感度具有重要临床意义。挖掘TCGA数据库中肺腺癌TPM标准化的表达矩阵,利用生物信息学方法研究DIP2A对肺腺癌(LUAD)的影响。结果显示,DIP2A在LUAD中高表达;差异基因分析结果显示共有829个差异基因(DEGs),其中DIP2A高表达组上调基因813个,下调基因为13个,GO和KEGG分析表明,这些差异基因涉及翻译活性和miRNAs在癌症中的发生。药物敏感性分析筛选出IC_(50)与DIP2A表达负相关的11种药物,包括阿法替尼、阿培利司、PIM抑制剂、Dot1l抑制剂、林西替尼、马来酰亚胺类似物、BCL-2抑制剂、奈拉滨、奥希替尼、索拉非尼以及极光激酶抑制剂。这些药物可能对DIP2A高表达肺腺癌具有一定的抑制作用。DIP2A is clinically important for the mechanism of action and anticancer drug sensitivity in lung adenocarcinoma.In this study,we mined the TPM-normalized expression matrix of lung adenocarcinoma in the TCGA database,and investigated the effect of this gene on lung adenocarcinoma(LUAD)using bioinformatics methods.The results showed that DIP2A was highly expressed in LUAD;differential gene analysis showed a total of 829 differential genes(DEGs),of which 813 genes were up-regulated and 13 genes were down-regulated in the DIP2A high-expression group,and GO and KEGG analyses indicated that these differential genes were involved in translational activity and miRNAs in cancer.Drug sensitivity analysis screens 11 drugs with negative correlation between IC_(50) and DIP2A expression,including afatinib,apelalis,PIM inhibitors,Dot1l inhibitors,lincitinib,maleimide analogs,BCL-2 inhibitors,nelarabine,ositinib,sorafenib,and aurora kinase inhibitors.These drugs may have some inhibitory effect on DIP2A-overexpressing lung adenocarcinomas.

关 键 词：DIP2A 肺腺癌 生物信息学分析 药物敏感度分析 

分 类 号：Q789[生物学—分子生物学]