饮食干预对妊娠期糖尿病小鼠子代糖尿病易感性的影响  

Effect of dietary intervention on the susceptibility of offspring diabetes in gestational diabetes mellitus mice

作　　者：林静 毛榕榕 闵颖俊 李通芬 金会艳 LIN Jing;MAO Rongrong;MIN Yingjun;LI Tongfen;JIN Huiyan(No.2 Clinical Medical College,Kunming Medical University,Kunming 650106;Pathology and Pathophysiology Department,Kunming Medical University,Kunming 650500;Pediatrics Department,the Sixth Affiliated Hospital of Kunming Medical University,Yuxi 653100;Functional Laboratory of Basic Medical Experimental Teaching Center,Kunming Medical University,Kunming 650500,China)

机构地区：[1]昆明医科大学第二临床医学院,云南昆明650106 [2]昆明医科大学病理学与病理生理学系,云南昆明650500 [3]昆明医科大学第六附属医院儿科,云南玉溪653100 [4]昆明医科大学基础医学院实验教学中心机能学实验室,云南昆明650500

出　　处：《临床医学研究与实践》2024年第7期32-36,共5页Clinical Research and Practice

基　　金：2020年度云南省科技厅-昆明医科大学应用基础研究联合专项资金项目(No.202001AY070001-016);昆明医科大学2020年省级大学生创新训练计划项目(No.202010678049);昆明医科大学大学生创新性实验项目(No.2020JXD022)。

摘　　要：目的探究饮食干预对妊娠期糖尿病(GDM)小鼠子代糖尿病易感性的影响。方法选取72只3周龄健康雌性小鼠,并将其随机均分成GDM组和低脂(LF)组。GDM组以饲喂高脂(HF)饲料建立GDM小鼠模型,LF组则饲喂LF饲料。两组小鼠的子代在3周龄断奶后进行饮食干预,分别饲喂HF饲料和LF饲料,并分为GDM HF(G-HF)组、GDM LF(G-LF)组、LF HF(L-HF)组、LF LF(L-LF)组。干预后测其体重、空腹血糖(FBG),并进行胰岛素耐受性试验(ITT)。结果HF饮食诱导建立的GDM小鼠模型成模率为33.33%,此模型发病过程与人类患GDM的病理生理发展过程较为相似。饮食干预到6周时,G-HF组的体重高于其他三组,且L-HF组体重高于L-LF组(P<0.05);饮食干预到12周时,G-HF组体重高于其他三组(P<0.05)。饮食干预到6周时,G-HF组的FBG水平高于G-LF组、L-LF组,L-HF组的FBG水平高于L-LF组(P<0.05);饮食干预到12周时,G-HF组的FBG水平高于其他三组,L-HF组的FBG水平高于L-LF组(P<0.05)。7周龄时,L-LF组对胰岛素的敏感性要高于G-HF组。结论GDM子代有明显的糖代谢紊乱及体重增加,且LF饮食可降低GDM子代患糖尿病的风险。Objective To explore the effect of dietary intervention on the susceptibility of offspring diabetes in gestational diabetes mellitus(GDM)mice.Methods Seventy-two 3-week-old healthy female mice were selected and randomly divided into GDM group and low-fat(LF)group.The GDM group was fed with high-fat(HF)diet to establish a GDM mouse model,and the LF group was fed with LF diet.The offspring of the two groups of mice were fed with HF diet and LF diet after weaning at 3 weeks of age,and were divided into GDM HF(G-HF)group,GDM LF(G-LF)group,LF HF(L-HF)group and LF LF(L-LF)group.After intervention,the body weight and fasting blood glucose(FBG)were measured,and insulin tolerance test(ITT)was conducted.Results The success rate of GDM mouse model induced by HF diet was 33.33%,the pathogenesis of this model was similar to the pathophysiological development of human GDM.At 6 weeks of dietary intervention,the body weight of the G-HF group was higher than that of the other three groups,and the body weight of the L-HF group was higher than that of the L-LF group(P<0.05);after 12 weeks of diet intervention,the body weight of the G-HF group was higher than that of the other three groups(P<0.05).After 6 weeks of diet intervention,the FBG level of the G-HF group was higher than that of the G-LF group and the L-LF group,and the FBG level of the L-HF group was higher than that of the L-LF group(P<0.05);at 12 weeks of diet intervention,the FBG level of the G-HF group was higher than that of the other three groups,and the FBG level of the L-HF group was higher than that of the L-LF group(P<0.05).At 7 weeks of age,the sensitivity of the L-LF group to insulin was higher than that of the G-HF group.Conclusion GDM offspring have obvious glucose metabolism disorder and weight gain,and LF diet can reduce the risk of diabetes in GDM offspring.

关 键 词：饮食干预 妊娠期糖尿病 体重 空腹血糖 胰岛素耐受性试验 

分 类 号：R587.1[医药卫生—内分泌]