谷胱甘肽硫转移酶基因多态性与汉族育龄女性胚胎停育易感性的关系  

Association between glutathione S-transferase polymorphisms and embryo damage among Han women of childbearing age

作　　者：姜玉婵 刘晓玲 李珍 鲁衍强 JIANG Yu-chan;LIU Xiao-ling;LI Zhen;LU Yan-qiang(The Second Maternal and Child Health Care Hospital of Ji'nan City,Ji'nan,Shandong 271100,China;不详)

机构地区：[1]济南市第二妇幼保健院,山东济南271100 [2]上海张江普汇转化医学研究院

出　　处：《中国妇幼保健》2024年第4期730-734,共5页Maternal and Child Health Care of China

基　　金：中国妇幼保健协会项目(MHBD-0634-001-MBN)。

摘　　要：目的探讨分析谷胱甘肽硫转移酶(GST)M1、GSTT1及GSTP13种基因的多态性与汉族育龄女性胚胎停育易感性的关系。方法采用病例-对照研究方法,选取2019年7月—2020年11月在济南市第二妇幼保健院就诊的育龄女性320例为研究对象,其中胚胎停育组125例,对照组195例。以受试者口腔黏膜上皮细胞为样本,提取基因组DNA,检测获取GSTM1、GSTT1及GSTP1基因多态性结果。统计分析GST基因多态性分布与胚胎停育发生的相关性。结果病例组GSTM1基因缺失型频率(65.6%)高于对照组(48.7%),差异有统计学意义(χ^(2)=8.783,P<0.05)。该基因缺失型是胚胎停育易感性的危险因素(OR=2.01,95%CI=1.26~3.19)。病例组GSTT1基因缺失型频率(55.2%)高于对照组(31.8%),差异有统计学意义(χ^(2)=17.256,P<0.05)。该基因的缺失型是胚胎停育易感性的危险因素(OR=2.63,95%CI=1.66~4.20)。对GSTM1、GSTT1基因联合分析后发现,2个基因中有1个及以上为缺失型,与2个基因均为非缺失型相比,两组胚胎停育发生率比较,差异有统计学意义(P<0.05)。GSTM1(+)/GSTT1(-)基因型的育龄女性发生胚胎停育的危险性是GSTM1(+)/GSTT1(+)基因型的2.68倍,其95%CI=1.29~5.59;GSTM1(-)/GSTT1(+)基因型的育龄女性发生胚胎停育的危险性是GSTM1(+)/GSTT1(+)基因型的2.04倍,其95%CI=1.06~3.90。当2个基因均为缺失型时,胚胎停育的发生风险进一步增加,GSTM1(-)/GSTT1(-)的育龄女性发生胚胎停育的危险性是GSTM1(+)/GSTT1(+)的5.37倍,其95%CI=2.70~10.67。对照组GSTP1 A313G位点AA、AG及GG基因型分布频率分别为67.7%、27.7%及4.6%,病例组分别为53.6%、42.4%及4.0%,两组基因型分布频率比较,差异有统计学意义(χ^(2)=7.430,P<0.05)。对照组中A等位基因的分布频率为81.5%,G等位基因的分布频率为18.5%,病例组中A等位基因分布频率为74.8%,G等位基因分布频率为25.2%,两组基因A、G等位基因频率分布比较,差异有统计学意义(χ^(2)=4.160,P<0.05)�Objective To investigate the association between polymorphisms of glutathione S-transferase(GST)(GSTM1,GSTT1,GSTP1)and embryo damage in Han women of reproductive age.Methods A case control study method was used to select 320 women of childbearing agewho visited the Second Maternal and Child Health Hospital of Ji'nan City during the same period,including 125 cases and 195 controls.Oral mucosal epithelial cells were collected to extract genomic DNA.GSTM1,GSTT1 and GSTP1 gene polymorphisms were detected by quantitative real-time PCR method,the results were analyzed statistically.Results The frequency of GSTM1 gene deletion in the case group(65.6%)was higher than that in the control group(48.7%).There was significant difference between two groups(χ^(2)=8.783,P<0.05).The deletion type of this gene was a risk factor for embryo damage(OR=2.01,95%CI=1.26-3.19).The frequency of GSTT1 gene deletion in the case group(55.2%)was higher than that in the control group(31.8%).There was significant difference between two groups(χ^(2)=17.256,P<0.05).The deletion type of this gene was a risk factor for embryo damage(OR=2.63,95%CI=1.66-4.20).After joint analysis of GSTM1 and GSTT1 genes,it was found that there was significant difference between the genotype with both genes being non-deletion and other genotype(P<005).The risk of embryo damage in GSTM1(+)/GSTT1(-)genotype was 2.68 timeshigher than that of GSTM1(+)/GSTT1(+)genotype(95%CI=1.29-5.59).The risk of embryo damage in GSTM1(-)/GSTT1(+)genotype was 2.04 timeshigher than that of GSTM1(+)/GSTT1(+)genotype(95%CI=1.06-3.90).When both genes were deletions,the risk of embryo damage further increases.The risk of embryo damage in GSTM1(-)/GSTT1(-)genotype was 5.37 times higher than that of GSTM1(+)/GSTT1(+)genotype(95%CI=2.70-10.67).The distribution frequencies of GSTP1 AA,AG,and GG genotypes in the control group were 67.7%,27.7%and 4.6%.The distribution frequencies of GSTP1 AA,AG,and GG genotypes in the case group were 53.6%,42.4%and 4.0%.The difference in genotype distribution fre

关 键 词：谷胱甘肽硫转移酶 基因多态性 胚胎停育 

分 类 号：R711[医药卫生—妇产科学]