濒危药用植物掌裂兰根部内生真菌和根际土真菌多样性分析  

Diversity of fungi in the roots and rhizosphere soil of the endangered medicinal plant Dactylorhiza hatagirea

作　　者：阿拉坦存布尔 曹乌吉斯古楞 包金花[3] 胡红霞 查苏娜 琪波热 Alatancunbuer;Caowujisiguleng;BAO Jinhua;HU Hongxia;CHA Suna;Qibore(Mongolian Medical College,Inner Mongolia Minzu University,Tongliao 028000,Inner Mongolia,China;Key Laboratory of Mongolian Medicine Research and Development Engineering,Ministry of Education,Inner Mongolia Minzu University,Tongliao 028000,Inner Mongolia,China;College of Agriculture,Inner Mongolia Minzu University,Tongliao 028000,Inner Mongolia,China)

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028000 [2]内蒙古民族大学蒙医药研发工程教育部重点实验室,内蒙古通辽028000 [3]内蒙古民族大学农学院,内蒙古通辽028000

出　　处：《微生物学通报》2024年第2期460-470,共11页Microbiology China

基　　金：内蒙古自治区科技计划(2021GG0003);内蒙古民族大学博士启动基金(BS509)。

摘　　要：【背景】植物内生真菌对宿主植物促生长、抗旱和增强抗病能力等方面有着重大的研究和利用价值,尤其对兰科植物的生长起到重要的作用。【目的】通过对掌裂兰根部内生真菌和根际土真菌多样性进行系统分析,掌握掌裂兰根部内生真菌与根际土真菌群落结构,为进一步探究掌裂兰植物与真菌共生规律提供参考。【方法】采用Illumina MiSeq高通量测序技术分析掌裂兰根部内生真菌和根际土真菌多样性。【结果】掌裂兰根部内生真菌隶属于7门89属,优势菌属为瘤菌根菌属(Epulorhiza)(16.93%)、头梗霉属(Cephaliophora)(10.41%)、酵母属(Saccharomyces)(5.73%)、角担菌属(Ceratobasidium)(5.32%)和镰刀菌属(Fusarium)(5.12%),其中Epulorhiza和Ceratobasidium为兰科植物菌根真菌;根际土真菌隶属于11门269属,优势菌属为镰刀菌属(Fusarium)(8.09%)、丛赤壳属(Neonectria)(6.79%)、Plectosphaerella(3.39%)和被孢霉属(Mortierella)(3.01%)。通过比较可知,掌裂兰根际土真菌的多样性明显高于根部内生真菌,存在明显差异。【结论】揭示了内蒙古地区掌裂兰根部内生真菌和根际土真菌类群组成,丰富了掌裂兰内生真菌资源,为推动资源紧缺型药用植物内生微生物研究及野生资源保护与开发发挥了重要作用。[Background]Endophytic fungi capable of promoting plant growth,enhancing plant tolerance to drought,and improving plant resistance to diseases have significant research and utilization values,especially for the growth of orchid plants.[Objective]To systematically analyze the diversity and reveal the community structure of fungi in the roots and rhizosphere soil of Dactylorhiza hatagirea,so as to provide a reference for further exploring the associations between D.hatagirea and fungi.[Methods]The Illumina Miseq high-throughput sequencing was employed to analyze the diversity of fungi in the roots and rhizosphere soil of D.hatagirea.[Results]The endophytic fungi in the roots of D.hatagirea belonged to 89 genera of 7 phyla,with the dominant genera being Epulorhiza(16.93%),Cephaliophora(10.41%),Saccharomyces(5.73%),Ceratobasidium(5.32%),and Fusarium(5.12%),among which Epulorhiza and Ceratobasidium were mycorrhizal fungi in orchid plants.The fungi in the rhizosphere soil belonged to 269 genera of 11 phyla,with the dominant genera being Fusarium(8.09%),Neonectria(6.79%),Plectosphaerella(3.39%),and Mortierella(3.01%).The diversity of fungi in the rhizosphere soil of D.hatagirea was significantly higher than that in the roots.[Conclusion]This study revealed the fungal composition in the roots and rhizosphere soil of D.hatagirea in Inner Mongolia,enriching the endophytic fungi resources of this medicinal plant.The findings help to promote the research on endophytic microorganisms in the medicinal plants with limited resources,alleviate the shortage of Chinese medicinal herb resources,and protect and reasonably develop wild medicinal plant resources.

关 键 词：掌裂兰 根部内真菌 根际土真菌 多样性 

分 类 号：S567.239[农业科学—中草药栽培] S154.3[农业科学—作物学]