Characterization of PetM cytochrome b6f subunit 7 domain-containing protein in tomato  

作　　者：Mustafa Bulut Adriano Nunes-Nesi Alisdair R.Fernie Saleh Alseekh 

机构地区：[1]Root Biology and Symbiosis,Max-Planck-Institute of Molecular Plant Physiology,Am Mühlenberg 1,14476 Potsdam,Germany [2]Departamento de Biologia Vegetal,Universidade Federal de Viçosa,Viçosa 36570-900 MG,Brazil [3]Plant Metabolomics,The Center for Plant Systems Biology and Biotechnology,4000 Plovdiv,Bulgaria

出　　处：《Horticulture Research》2023年第12期14-25,共12页园艺研究（英文）

基　　金：We would like to thank Dr.Micha Wijesingha Ahchige for guiding and giving advice for the CRISPR/Cas9 vector generation and Dr.Mark A.Schoettler and Dr.Ryo Yokohama for the scientific advices and discussions.Also thanks to Dr.Karin Köhl,the greenhouse team of theMax Planck Institute of Molecular Plant Physiology,for transforming and handling the plants.M.B.appreciates the finan-cial support of the International Max Planck Research School for Molecular Plant Sciences(IMPRS-MolPlant).The research fellow-ship granted by Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq-Brazil)to A.N.-N.is gratefully acknowledged.A.R.F.and S.A.acknowledge the European Union’s Horizon 2020 research and innovation programme,project PlantaSYST(SGA-CSA No.739582 under FPA No.664620);the BG05M2OP001-1.003-001-C01 project;financed by the European Regional Devel-opment Fund through the Bulgarian’Science and Education for Smart Growth’Operational Programme.S.A.acknowledges the EU Horizon 2020,call HORIZON-WIDERA-2022-TALENTS-01,project NatGenCrop(grant agreement No.101087091).

摘　　要：In recent years,multiple advances have been made in understanding the photosynthetic machinery in model organisms.Knowledge transfer to horticultural important fruit crops is challenging and time-consuming due to restrictions in gene editing tools and prolonged life cycles.Here,we characterize a gene encoding a PetM domain-containing protein in tomato.The CRISPR/Cas9 knockout lines of the PetM showed impairment in the chloroplastic electron transport rate(ETR),reduced CO_(2) assimilation,and reduction of carotenoids and chlorophylls(Chl)under several light conditions.Further,growth-condition-dependent elevation or repression of Chl a/b ratios and de-epoxidation states were identified,underlining possible impairment compensation mechanisms.However,under low light and glasshouse conditions,there were basal levels in CO_(2) assimilation and ETR,indicating a potential role of the PetM domain in stabilizing the cytochrome b6f complex(Cb6f)under higher light irradiance and increasing its quantum efficiency.This suggests a potential evolutionary role in which this domain might stabilize the site of the Cb6f regulating ratios of cyclic and linear electron transport and its potential importance during the conquest of terrestrial ecosystems during which plants were exposed to higher irradiance.Finally,the results are discussed with regard to metabolism and their implication to photosynthesis from an agronomic perspective.

关 键 词：ASSIMILATION METABOLISM consuming 

分 类 号：S641.2[农业科学—蔬菜学]