Berry texture QTL and candidate gene analysis in grape(Vitis vinifera L.)  

作　　者：Hong Lin Li Ma Qiuyu Guo Cheng Liu Yangming Hou Zhendong Liu Yuhui Zhao Changyue Jiang Xiuwu Guo Yinshan Guo 

机构地区：[1]College of Horticulture,Shenyang Agricultural University,120 Dongling Road,Shenyang,110866,China [2]Ministry of Education Key Laboratory of Protected Horticulture,Shenyang Agricultural University,Shenyang,110866,China

出　　处：《Horticulture Research》2023年第12期152-164,共13页园艺研究（英文）

基　　金：The work was supported by the National Natural Science Foundation of China(No.32102317 and No.31972368);the China Agriculture Research System(No.CARS-29-yc-6);the China Postdoctoral Science Foundation(No.2021 M693866);the Department of Science and Technology of Liaoning Province(No.2022030723-JH5/104);the Shenyang Science and Technology Bureau Funds(No.21-116-3-27);the Liaoning key R&D Program(No.2020JH2/10200032).

摘　　要：Berry texture is a noteworthy economic trait for grape;however,the genetic bases and the complex gene expression and regulatory mechanism for the diverse changes in berry texture are still poorly understood.In this study,the results suggest that it is difficult to obtain high-mesocarp firmness(MesF)and high-pericarp puncture hardness(PPH)grape cultivars with high pericarp brittleness(PerB).The high-density linkage map was constructed using whole-genome resequencing based on 151 F1 individuals originating from intraspecific hybridization between the firm-flesh cultivar‘Red Globe’and soft-flesh cultivar‘Muscat Hamburg’.The total length of the consensus map was 1613.17 cM,with a mean genetic distance between adjacent bin markers of 0.59 cM.Twenty-seven quantitative trait loci(QTLs)for berry MesF,PPH,and PerB were identified in linkage groups(LGs)1,3,4,6,8,9,10,11,14,16,and 17,including twelve QTLs that were firstly detected in LGs 6,11,and 14.Fourteen promising candidate genes were identified from the stable QTL regions in LGs 10,11,14,and 17.In particular,VvWARK2 and VvWARK8 refer to chromosome 17 and are two promising candidate genes for MesF and PPH,as the VvWARK8 gene may increase pectin residue binding with WARK for high berry firmness maintenance and the allele for VvWARK2 carrying the‘CC’and‘GA’genotypes at Chr17:1836764 and Chr17:1836770 may be associated with non-hard texture grape cultivars.In addition,real-time quantitative polymerase chain reaction(RT-qPCR)verification revealed that the promising candidate transcription factor genes VvMYB4-like,VvERF113,VvWRKY31,VvWRKY1,and VvNAC83 may regulate cell wall metabolism candidate gene expression for grape berry texture changes.

关 键 词：CULTIVAR FIR BERRY 

分 类 号：S663.1[农业科学—果树学]