金银花对金黄色葡萄球菌体外抑菌作用研究  

Study on antibacterial effect of Lonicera Japonica Thunb on Staphylococcus aureus in vitro

作　　者：易雪丽[1] 陈晓颖 韦家柱 陈启威[1] 庞艳芳 Yi Xueli;Chen Xiaoying;Wei Jiazhu;Cheng Qiwei;Pang Yanfang(Department of Clinical Laboratories,The Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院附属医院检验科,广西百色533000

出　　处：《右江民族医学院学报》2024年第1期48-51,共4页Journal of Youjiang Medical University for Nationalities

基　　金：百色市本级科学研究与技术开发计划项目(百科20211808)。

摘　　要：目的比较金银花对甲氧西林敏感金黄色葡萄球菌(MSSA)和甲氧西林耐药金黄色葡萄球菌(MRSA)体外抑菌活性差异。方法收集30株MSSA和30株MRSA菌,使用头孢西丁筛选试验和mecA基因检测对菌株进行验证,使用琼脂打孔法检测金银花对两类金黄色葡萄球菌的抑菌活性并比较差异,使用琼脂稀释法检测金银花对两类金黄色葡萄球菌的MIC并计算出MIC_(50)。结果金银花对MSSA和MRSA均有体外抑菌活性且MIC_(50)均为3.125 mg/mL。结论金银花对MSSA和MRSA均有较好的体外抑菌活性,在临床治疗金黄色葡萄球菌感染方面有很大的应用前景。Objective To compare the difference of antibacterial activity of Lonicera Japonica Thunb against Methicillin-sensitive Staphylococcus aureus(MSSA)and Methicillin-resistant Staphylococcus aureus(MRSA)in vitro.Methods Thirty strains of MSSA and MRSA were collected and identified by Cefoxitin screening test and mecA gene detection,respectively.The agar punch method was used to detect the antibacterial activity of Lonicera Japonica Thunb against two types of Staphylococcus aureus and compared their differences.Agar dilution method was used to detect the MIC of Lonicera Japonica Thunb against two types of Staphylococcus aureus,and the MIC_(50) was calculated.Results Lonicera Japonica Thunb had in vitro antibacterial activity against both MSSA and MRSA with MIC_(50) of 3.125 mg/mL.Conclusion Lonicera Japonica Thunb has a good antibacterial activity against both MSSA and MRSA in vitro,and has great application prospect in the clinical treatment of Staphylococcus aureus infection.

关 键 词：金银花 金黄色葡萄球菌 甲氧西林敏感金黄色葡萄球菌 甲氧西林耐药金黄色葡萄球菌 

分 类 号：R285[医药卫生—中药学]