Dectin-1对侵袭性肺曲霉病大鼠半乳甘露聚糖、胸腺细胞凋亡及肺损伤的影响  

Effects of Dectin-1 on galactomannan,thymocyte apoptosis and lung injury in invasive pulmonary aspergillosis rats

作　　者：邓浩然[1] 丁旭春[1] Deng Haoran;Ding Xuchun(Department of Respiratory and Critical Care Medicine,Hangzhou Hospital of Traditional Chinese Medicine Affiliated to Zhejiang University of Traditional Chinese Medicine,Hangzhou 310007,China)

机构地区：[1]浙江中医药大学附属杭州市中医院呼吸与危重症医学科,杭州310007

出　　处：《解剖学杂志》2023年第6期508-513,共6页Chinese Journal of Anatomy

基　　金：杭州市医药卫生科技项目(20211231Y064)。

摘　　要：目的:探究树突状细胞相关性C型凝集素-1(Dectin-1)对侵袭性肺曲霉病大鼠半乳甘露聚糖(GM)、胸腺细胞凋亡及肺损伤的影响。方法:大鼠随机分为对照组、Ad-EGFP组和Ad-Dectin-1-EGFP组。检测大鼠肺功能(肺容积变换、静息通气量);ELISA法检测大鼠肺泡灌洗液中干扰素γ(IFN-γ)、白细胞介素4(IL-4)含量和血清中GM含量;H-E染色观察肺组织病理学变化;检测胸腺指数;TUNEL检测胸腺细胞凋亡;免疫印迹检测肺组织中Dectin-1、T-bet、GATA-3蛋白表达。结果:与对照组相比,Ad-EGFP组大鼠IFN-γ含量、胸腺指数、肺容积变换和静息通气量均明显降低,IL-4含量、GM值、胸腺细胞凋亡指数明显升高;与Ad-EGFP组相比,Ad-Dectin-1-EGFP组大鼠IFN-γ含量、胸腺指数、肺容积变换和静息通气量均明显升高,IL-4含量、GM值、胸腺细胞凋亡指数明显降低。与对照组相比,Ad-EGFP组大鼠肺组织中Dectin-1、T-bet蛋白表达明显降低,GATA-3蛋白表达明显升高;与Ad-EGFP组相比,Ad-Dectin-1-EGFP组大鼠肺组织中Dectin-1、T-bet蛋白表达明显升高,GATA-3蛋白表达明显降低。结论:Dectin-1重组质粒可减轻侵袭性肺曲霉病大鼠肺损伤,降低血清中GM值,通过抑制胸腺细胞凋亡维持体内免疫功能平衡,其机制可能与T-bet、GATA-3蛋白表达有关。Objective:To investigate the effect of dendritic cell associated C-type lectin-1(Dectin-1)on galactomannan(GM),thymocyte apoptosis and lung injury in invasive pulmonary aspergillosis rats.Methods:Thirty rats were randomly divided into control group,Ad-EGFP group and Ad-Dectin-1-EGFP group.The lung function of rats(transformation of lung volume and resting breath)was detected.The contents of interferon-γ(IFN-γ)and interleukin-4(IL-4)in bronchoalveolar lavage fluid and the content of GM in serum were detected by ELISA.The pathological changes of lung tissue were detected by H-E staining.Thymus index was examined.TUNEL was used to detect thymocyte apoptosis.Western blotting was used to detect the expression of Dectin-1,T-bet,and GATA-3 protein in lung tissue.Results:Compared with in the control group,the IFN-γcontent,thymus index,lung volume change and resting ventilation volume were significantly decreased,while the IL-4 content,GM value and apoptotic index,the thymus index were significantly increased in the Ad-EGFP group.Compared with the Ad-EGFP group,the content of IFN-γ,the thymus index,the lung volume change and the resting ventilation volume were significantly increased in the Ad-Dectin-1-EGFP group,while the content of IL-4,GM value and apoptosis index were decreased significantly.Compared with the control group,the expressions of Dectin-1 and T-bet proteins in the lung tissue of the Ad-EGFP group were significantly decreased,and the expression of GATA-3 protein was significantly increased.Compared with the Ad-EGFP group.The expressions of Dectin-1 and T-bet proteins in lung tissue of rats in the Ad-Dectin-1-EGFP group were significantly increased,and the expression of GATA-3 protein was significantly decreased.Conclusion:Dectin-1 recombinant plasmid can alleviate lung injury in invasive pulmonary aspergillosis rats,reduce serum GM value,and maintain the balance of immune function by inhibiting thymocyte apoptosis.The mechanism may be related to the expression of T-bet and GATA-3 proteins.

关 键 词：树突状细胞相关性C型凝集素1 侵袭性肺曲霉病 半乳甘露聚糖 胸腺细胞 凋亡 肺损伤 大鼠 

分 类 号：R519[医药卫生—内科学]