Sclerostin antibody improves alveolar bone quality in the Hyp mouse model of X-linked hypophosphatemia  

作　　者：Kelsey A.Carpenter Delia O.Alkhatib Bryan A.Dulion Elizabeth Guirado Shreya Patel Yinghua Chen Anne George Ryan D.Ross 

机构地区：[1]Department of Anatomy&Cell Biology,Rush University Medical Center,Chicago,IL,USA [2]Department of Oral Biology,The University of Illinois at Chicago,Chicago,IL,USA [3]Department of Orthopedic Surgery,Rush University Medical Center,Chicago,IL,USA [4]Department of Microbial Pathogens and Immunity,Rush University Medical Center,Chicago,IL,USA

出　　处：《International Journal of Oral Science》2023年第4期640-648,共9页国际口腔科学杂志（英文版）

基　　金：supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases and the National Institute of Dental and Craniofacial Research of the National Institute of Health under award numbers K01 AR073923 and R03DE029873,respectively

摘　　要：X-linked hypophosphatemia (XLH) is a rare disease of elevated fibroblast growth factor 23 (FGF23) production that leads tohypophosphatemia and impaired mineralization of bone and teeth. The clinical manifestations of XLH include a high prevalence ofdental abscesses and periodontal disease, likely driven by poorly formed structures of the dentoalveolar complex, including thealveolar bone, cementum, dentin, and periodontal ligament. Our previous studies have demonstrated that sclerostin antibody (SclAb) treatment improves phosphate homeostasis, and increases long bone mass, strength, and mineralization in the Hyp mousemodel of XLH. In the current study, we investigated whether Scl-Ab impacts the dentoalveolar structures of Hyp mice. Male andfemale wild-type and Hyp littermates were injected with 25 mg·kg−1 of vehicle or Scl-Ab twice weekly beginning at 12 weeks of ageand euthanized at 20 weeks of age. Scl-Ab increased alveolar bone mass in both male and female mice and alveolar tissue mineraldensity in the male mice. The positive effects of Scl-Ab were consistent with an increase in the fraction of active(nonphosphorylated) β-catenin, dentin matrix protein 1 (DMP1) and osteopontin stained alveolar osteocytes. Scl-Ab had no effecton the mass and mineralization of dentin, enamel, acellular or cellular cementum. There was a nonsignificant trend towardincreased periodontal ligament (PDL) attachment fraction within the Hyp mice. Additional PDL fiber structural parameters were notaffected by Scl-Ab. The current study demonstrates that Scl-Ab can improve alveolar bone in adult Hyp mice.

关 键 词：ALVEOLAR IMPAIRED ELEVATED 

分 类 号：R596[医药卫生—内科学] R78[医药卫生—临床医学]