依福地平对猪流行性腹泻病毒的抑制作用研究  

Inhibition of efonidipine on porcine epidemic diarrhea virus

作　　者：张财盛 陈欢 司安琪 赵普 齐传翔 钱莺娟[1] 郑龙三 戴建君[1] ZHANG Caisheng;CHEN Huan;SI Anqi;ZHAO Pu;QI Chuanxiang;QIAN Yingjuan;JUNG Yongsam;DAI Jianjun(College of Veterinary Medicine,Nanjing Agricultural University/MOE Joint International Research Laboratory of Animal Health and Food Safety/Key Laboratory of Bacteriology,Ministry of Agriculture and Rural Affairs,Nanjing 210095,China)

机构地区：[1]南京农业大学动物医学院/教育部动物健康与食品安全国际合作联合实验室/农业农村部细菌学重点实验室,江苏南京210095

出　　处：《畜牧与兽医》2024年第3期62-68,共7页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(U19A2039,31900141);中央高校基本业务费项目(KJQN202022);江苏省高校优势学科建设工程项目(PAPD)。

摘　　要：猪流行性腹泻病毒(porcine epidemic diarrhea virus, PEDV)是猪流行性腹泻(PED)的病原。本研究通过CCK-8、细胞ELISA、Western blot、时间过程分析、药物抑制病毒吸附和入胞试验,探究了依福地平对PEDV的抑制作用。结果表明,依福地平在20μmol/L浓度范围内,对Vero-E6和Marc-145细胞毒性较低;Western blot结果表明,依福地平能够显著降低宿主细胞中PEDV-N蛋白水平,主要抑制PEDV感染的入胞阶段。研究还发现,依福地平能抑制Ⅰ型单纯疱疹病毒和猪伪狂犬病毒感染宿主细胞,是一类潜在的广谱抗病毒药物。Porcine epidemic diarrhea virus(PEDV)is the causative agent of porcine epidemic diarrhea(PED).In this study,CCK-8,Cell-based ELISA,Western blot,Time-of-addition assay and viral binding and entry assay were performed to determine the inhibitory effect and molecular mechanism of efonidipine on PEDV infection.The results showed that efonidipine had low cytotoxicity on Vero-E6 and Marc-145 cells within the concentration of 20μmol/L.Meanwhile,the Western blot results showed that efonidipine significantly inhibited the ex-pression level of the PEDV-N protein on the susceptible cells,mainly inhibiting the entry stage of PEDV.Also,efonidipine was able to in-hibit the infection of host cells with herpes simplex virus typeⅠ(HSV-1)and porcine pseudorabies virus(PRV),making it a potential broad-spectrum antiviral drug.

关 键 词：猪流行性腹泻病毒 依福地平 病毒入胞 广谱抗病毒效应 

分 类 号：S855[农业科学—临床兽医学]