水貂病毒性肠炎杆状病毒载体灭活疫苗与水貂犬瘟热活疫苗混合免疫效果评价  

Evaluation of the combined immune efficacy of inactivated mink viral enteritis baculovirus vector vaccine and live mink canine distemper vaccine

作　　者：吴洪超 霍宁宁 丁航天 曹玉姣 陈亚磊 陈云豫 王璐璐 刘玉秀 田克恭 WU Hongchao;HUO Ningning;DING Hangtian;CAO Yujiao;CHEN Yalei;CHEN Yunyu;WANG Lulu;LIU Yuxiu;TIAN Kegong(College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450000,China;National Research Center for Veterinary Medicine,Luoyang 471003,China;Luoyang Huizhong Biotechnology Co.,Ltd,Luoyang 471003,China;Pulike Biological Engineering Inc.,Ltd.,Luoyang 471003,China)

机构地区：[1]河南农业大学动物医学院,河南郑州450000 [2]国家兽用药品工程技术研究中心,河南洛阳471003 [3]洛阳惠中生物技术有限公司,河南洛阳471003 [4]普莱柯生物工程股份有限公司,河南洛阳471003

出　　处：《畜牧与兽医》2024年第3期106-112,共7页Animal Husbandry & Veterinary Medicine

基　　金：中原学者田克恭科学家工作室项目。

摘　　要：为评价水貂病毒性肠炎杆状病毒载体灭活疫苗(简称MEV亚单位疫苗)与水貂犬瘟热活疫苗(简称CDV活疫苗)混合免疫的效果,本研究将两种疫苗混合后在室温静置不同时间测定犬瘟热病毒(CDV)含量,并选用30只健康水貂随机分为6组进行比对试验,其中G1和G2组免疫MEV亚单位疫苗稀释CDV活疫苗的混合疫苗,G3组为CDV活疫苗单独免疫组,G4组为MEV亚单位疫苗单独免疫组,G5和G6组分别为CDV攻毒组和MEV攻毒组。免疫后21 d采血检测CDV中和抗体,同时对G1、G3、G5组进行CDV攻毒;免疫后14 d采血检测MEV血凝抑制(HI)抗体,同时对G2、G4、G6组进行MEV攻毒。结果:两种疫苗混合后在室温静置2 h, CDV含量无明显下降。免疫后21 d, G1和G3组CDV中和抗体效价达到1∶64.6~1∶128.8,CDV攻毒后混合疫苗和CDV活疫苗免疫组的保护率均为100%。免疫后14 d, G2和G4组的MEV HI抗体效价达到1∶128~1∶1 024,MEV攻毒后混合疫苗和MEV亚单位疫苗免疫组的保护率均为100%。研究表明,MEV亚单位疫苗稀释CDV活疫苗,混合免疫后各疫苗的免疫效力均不受影响。This study was to evaluate the combined immune efficacy of inactivated mink viral enteritis baculovirus vector vaccine(referred to as MEV subunit vaccine)and live mink canine distemper vaccine(referred to as CDV live vaccine).Here,MEV subunit vaccine was used to dilute CDV live vaccine.The diluted mixed vaccine was left at room temperature to determine the canine distemper virus(CDV)titer at different time points.The results showed that the CDV titers did not reduce within 2 hours post-dilution.Then,30 healthy mink were ob-tained and randomly divided into six groups for the experiment.10 minks in Group 1 and Group 2 were immunized with the mixed vaccine.5 minks in Group 3 were immunized with the live CDV vaccine.5 minks in Group 4 were immunized with the MEV subunit vaccine.Group 5 and Group 6 were the CDV and MEV challenged group,respectively.At 21 days after immunization,blood samples were collected from the animals to detect CDV neutralizing antibody.Groups 1,3 and 5 were challenged with the CDV virus.At 14 days after immunization,blood samples were collected to detect MEV HI antibody.Groups 2,4 and 6 were challenged with the MEV virus.The results showed that the titer of the CDV neutralizing antibody was 1∶646-1∶1288 in Groups 1 and 3 at 21 days after immunization.After CDV challenging,the pro-tection rate was 100%in both the mixed vaccine group and the live CDV vaccine group.The titer of the MEV HI antibody was 1∶128-1∶1024 in Groups 2 and 4 at 14 days after immunization.The protection rate against MEV challenge was 100%in both vaccine groups.These results indicated that MEV subunit vaccine could be used to dilute CDV live vaccine,and the immune efficacy of each vaccine was not affect-ed by combined vaccination.

关 键 词：水貂病毒性肠炎杆状病毒载体灭活疫苗 水貂犬瘟热活疫苗 混合免疫 免疫效力 

分 类 号：S858[农业科学—临床兽医学]