细胞糖酵解对猪流行性腹泻病毒复制的影响  

Effect of cellular glycolysis on PEDV replication

作　　者：吴冰 季霖 徐雅雯 唐泰山 杨龙圣 何召庆 范红结[1] 蔺辉星[1] WU Bing;JI Lin;XU Yawen;TANG Taishan;YANG Longsheng;HE Zhaoqing;FAN Hongjie;LIN Huixing(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Animal,Plant and Food Inspection Center,Nanjing Customs District,Nanjing 210019,China;Zhaofenghua Bio-Industry(Nanjing)Co.,Ltd.,Nanjing 211102,China)

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]南京海关动植物与食品检测中心,江苏南京210019 [3]兆丰华生物科技(南京)有限公司,江苏南京211102

出　　处：《畜牧与兽医》2024年第3期113-117,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划项目(2022YFD1800900);国家自然科学基金项目(31702278);大学生创新训练计划(SRT)项目(202317YX034、202217YX777);江苏高校优势学科建设工程专项资金项目(PAPD)。

摘　　要：为研究猪流行性腹泻病毒(PEDV)感染细胞后,细胞糖酵解的变化及其对PEDV复制的影响,本试验通过实时荧光定量PCR和Western blot检测PEDV感染猪小肠上皮细胞IPEC-J2后36 h糖酵解相关酶己糖激酶2(HK2)的表达水平,然后通过添加糖酵解抑制剂2-脱氧-D-葡萄糖(2-DG)来进一步验证细胞糖酵解对PEDV复制的影响,最后检测了正常浓度葡萄糖(5.5 mmol/L)和高浓度葡萄糖(11.1 mmol/L和22.2 mmol/L)培养条件下PEDV复制情况来探究葡萄糖浓度对PEDV复制的影响。结果表明,PEDV感染显著增加糖酵解限速酶HK2的表达,PEDV感染可增强IPEC-J2细胞的糖酵解,细胞糖酵解水平增强有利于PEDV的复制。在一定范围内提高葡萄糖浓度可促进PEDV复制。研究结果为初步阐明PEDV感染机制以及冠状病毒病的综合防控提供了理论依据。This study was to investigate the changes in glucose metabolism in IPEC-J2 cells infected with porcine epidemic diarrhea virus(PEDV)and the effect of cell glycolysis on PEDV replication.The level of glycolysis related enzyme Hexokinase 2(HK2)in IPEC-J2 cells at 36 h post PEDV infection was detected by quantitative PCR and Western blot.Then,the effect of cell glycolysis on PEDV replication was further identified by adding glycolysis inhibitor 2-Deoxy-D-glucose(2-DG).Finally,the replication of PEDV under the conditions of nor-mal concentration of glucose(55 mmol/L)and high concentration of glucose(111 mmol/L and 222 mmol/L)conditions was detected to investigate the effect of glucose concentration on PEDV replication.The results showed that PEDV infection significantly increased the expres-sion of the glycolysis rate limiting enzyme HK2,which means that PEDV infection could enhance the glycolysis in IPEC-J2 cells.The en-hanced level of cell glycolysis was conducive to the replication of PEDV.Increasing glucose concentration within a certain range could pro-mote the replication of PEDV.This research provided a theoretical basis for preliminary elucidation of the infection mechanism of PEDV and comprehensive prevention and control of coronaviruses.

关 键 词：猪流行性腹泻病毒 糖酵解 复制 

分 类 号：S852[农业科学—基础兽医学]