miR-429对烧伤小鼠肺组织炎性反应和氧化应激的影响  

Effect of miR-429 on inflammatory response and oxidative stress in lung tissue of burned mice

作　　者：张珂珂 黄国宝 ZHANG Keke;HUANG Guobao(School of Clinical Medicine Weifang Medical University,Shandong,Weifang 261053,China)

机构地区：[1]潍坊医学院临床医学院,潍坊医学院,山东省潍坊市261053

出　　处：《河北医药》2024年第3期330-334,共5页Hebei Medical Journal

基　　金：山东省优秀中青年科学家科研奖励基金(编号:BS2020SW1407)。

摘　　要：目的探讨miR-429对烧伤小鼠肺组织炎性反应和氧化应激的影响及可能的机制。方法24只C57BL/6小鼠,按随机数表法分为对照组、模型组、阴性对照组和miR-429抑制剂组,每组6只。除对照组外,其他3组小鼠通过热水烫伤法构建小鼠烧伤模型。其中,miR-429抑制剂组和阴性对照组小鼠通过尾静脉分别注射等量的miR-429抑制剂miR-429 antagomir以及阴性对照miR-NC;对照组和模型组小鼠给予等体积0.9%氯化钠溶液。qRT-PCR实验检测肺组织中miR-429的表达水平;ELISA法检测肺组织中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白细胞介素-6(IL-6)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH)水平;HE染色评估肺组织病理变化;TUNEL法检测肺组织细胞凋亡情况;Western blotting检测肺组织中Nrf2和HO-1蛋白表达情况。结果与对照组小鼠比较,模型组小鼠肺泡壁变宽,并出现明显的组织水肿和炎症细胞浸润;肺组织中凋亡细胞数量明显增多,且miR-429表达、TNF-α、IL-1β、IL-6和MDA含量明显升高,而SOD、CAT和GSH含量以及Nrf2蛋白、HO-1蛋白表达显著降低,差异均有统计学意义(P<0.05);与阴性对照组比较,miR-429抑制剂组小鼠肺组织病理损伤情况得到明显改善;肺组织中凋亡细胞数量明显减少,miR-429表达、TNF-α、IL-1β、IL-6和MDA含量明显降低,而SOD、CAT和GSH含量以及Nrf2蛋白、HO-1蛋白表达显著升高,差异均有统计学意义(P<0.05)。结论抑制miR-429表达能够抑制烧伤小鼠肺组织的炎性反应,减轻氧化应激损伤,其分子机制或许与Nrf2/HO-1信号通路相关。Objective To investigate the effect and possible mechanism of miR-429 on inflammatory response and oxidative stress in lung tissue of burned mice.Methods A total of 24 C57BL/6 mice were randomly divided into Control group,Model group,Negative control group and miR-429 inhibitor group,with 6 mice in each group.Except for Control group,the other three groups of mice were stimulated by hot water to establish a burned model.Before modeling,mice in the miR-429 inhibitor group and Negative control group were injected with the same amount of miR-429 antagomir or miR-NC through tail vein,respectively.Meanwhile,mice in Control group and Model group were given an equal volume of 0.9%sodium chloride solution.The expression level of miR-429 in lung tissue was detected by quantitative real-time polymerase chain reaction(qRT-PCR).The levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH)in lung tissues were detected by enzyme-linked immunosorbent assay(ELISA).Pathological changes of lung tissue were assessed by the hematoxylin and eosin(H&E)staining.Cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)staining.In addition,the related-protein expression associated to nuclear factor erythroid 2-related factor 2(Nrf2)/heme oxygenase-1(HO-1)pathway was measured by western blotting.Results Compared with Control group,the alveolar wall of the Model group widened,with significant tissue edema and inflammatory cell infiltration.The number of apoptotic cells in lung tissue significantly increased,miR-29 expression and the levels of TNF-α,IL-1β,IL-6 and MDA were significantly up-regulated(P<0.05).However,the levels of SOD,CAT and GSH and the protein expressions of Nrf2 and HO-1 decreased significantly(P<0.05).Compared with Negative control group,the pathological lesion of lung tissue in miR-429 inhibitor group was significantly mitigated.The number of apoptotic cells i

关 键 词：miR-429 烧伤 氧化应激 NRF2 HO-1 

分 类 号：R644[医药卫生—外科学]