小红参乙酸乙酯提取物调控PI3K/AKT/GSK3-β通路对心肌缺血再灌注损伤大鼠雌激素的影响  

Effects of ethyl acetate extract of Red ginseng on estrogen in rats with myocardial ischemia-reperfusion injury by regulating the PI3K/AKT/GSK3-βpathway

作　　者：张国庆 周艳华 王仙琪 王佳佳 ZHANG Guoqing;ZHOU Yanhua;WANG Xianqi(Xi’an Jiaotong University,Shaanxi,Xi’an 710000,China;不详)

机构地区：[1]西安交通大学,西安市710000 [2]西安中大国际医院全科医学科 [3]西北妇女儿童医院心脏中心 [4]空军军医大学第一附属医院西京医院急诊科 [5]空军军医大学第一附属医院西京医院麻醉与围术期医学科

出　　处：《河北医药》2024年第4期501-505,共5页Hebei Medical Journal

基　　金：国自然基金课题项目(编号:82201367)。

摘　　要：目的研究小红参乙酸乙酯提取物通过调控磷脂酰肌醇3-激酶(phosphatidylinositol-3-OH kinase,PI3K)/蛋白激酶B(protein kinase B,Akt)/糖原合成酶激酶3-β(glycogen synthase kinase 3-β,GSK3-β)通路对心肌缺血再灌注损伤大鼠雌激素的影响。方法选取SPF级SD雌性大鼠50只,空白组10只,建模中死亡10只,随机分为模型组、低剂量组、高剂量组,每组10只。对照组不做任何处理,模型组、低剂量组、高剂量组进行心肌缺血再灌注损伤模型建模,建模成功后模型组不做任何处理,低剂量组、高剂量组分别给予小红参乙酸乙酯提取物灌胃,其剂量分别为56.7 mg/kg、280 mg/kg。观察大鼠心肌损伤[肌钙蛋白I(CTnI)、肌酸激酶同工酶(CK-MB)]、氧化应激[丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)]、一氧化氮(NO)、内皮-氧化氮合酶(eNOS)含量、性激素、PI3K/AKT/GSK3-β信号通路表达量情况。结果与空白组比较,模型组、低剂量组、高剂量组cTnI、CK-MB、MDA、E2水平均升高(P<0.05),SOD、GSH-Px、NO、eNOS水平、PI3K、AKT、GSK3-β表达均降低(P<0.05);与模型组比较,低剂量组、高剂量组cTnI、CK-MB、MDA、E2水平均降低(P<0.05),SOD、GSH-PxNO、eNOS水平、PI3K、AKT、GSK3-β表达均升高(P<0.05);与低剂量组比较,高剂量组cTnI、CK-MBE2水平均降低(P<0.05),SOD、GSH-PxNO、eNOS水平、PI3K、AKT、GSK3-β表达均升高(P<0.05)。结论小红参乙酸乙酯提取物能对心肌缺血再灌注损伤大鼠能心肌损伤情况进行改善,抑制氧化应激状态,恢复雌激素水平,通过调节PI3K/AKT/GSK3-β通路能够反映出对心肌缺血再灌注损伤的干预效果。Objective To analyze the regulatory effect of ethyl acetate extract of Red ginseng on estrogen in myocardial ischemia-reperfusion injury rats through the phosphatidylinositol-3-OH kinase(PI3K)/protein kinase B(AKT)/glycogen synthase kinase 3-β(GSK3-β)signaling pathway.Methods Fifty female Sprague-Dawley(SD)rats in the specific pathogen-free(SPF)level were selected.Ten rats died during the modeling,and the remaining rats were randomly divided into blank group,model group,low-dose group,and high-dose group,with 10 rats peRgroup.Rats in the control group were treated with blank control,and the remaining were subjected to the modeling of myocardial ischemia-reperfusion injury.Rats in low-dose group and high-dose group were further given ethyl acetate extract of red ginseng at the dose of 56.7mg/kg and 280mg/kg by intragastric administration,respectively.Myocardial damage(troponin I[cTnI],creatine kinase isoenzyme[CK-MB]),oxidative stress(malondialdehyde[MDA],superoxide dismutase[SOD],plasma glutathione peroxidase[GSH-Px]),nitric oxide(NO),inducible nitric oxide synthase(eNOS),sex hormones,and expression levels of key proteins in the PI3K/AKT/GSK3-βsignaling pathway were detected.Results Compared with those of the blank group,the levels of cTnI,CK-MB,MDA,and estrogen(E2)in the model group,low-dose group,and high-dose group were all significantly higher,and the levels of SOD,GSH-Px,NO,eNOS,and protein expressions of PI3K,AKT and GSK3-βwere significantly lower (all P<0.05).Compared with those of the model group,the levels of cTnI,CK-MB,MDA,and E2 in the low-dose group and the high-dose group were significantly lower,and the levels of SOD,GSH-Px,NO,eNOS,and protein expressions of PI3K,AKT and GSK3-βwere significantly higher (all P<0.05).Compared with those of the low-dose group,the levels of cTnI and CK-MB and E2 in the high-dose group were significantly lower,and the levels of SOD,GSH-Px,NO,and eNOS and protein expressions of PI3K,AKT and GSK3-βexpression were significantly higher (all P<0.05).Conclusion Ethyl ace

关 键 词：心肌缺血再灌注 小红参乙酸乙酯 雌激素 磷脂酰肌醇3-激酶/蛋白激酶B/糖原合成酶激酶3-β 

分 类 号：R542.2[医药卫生—心血管疾病]