甜瓜MADS-box基因家族的鉴定及系统进化分析  

Identification and Phylogenetic Analysis of MADS-box Gene Family in Cucumis melo L.

作　　者：秦玉杰 任辉 祝建波[1] Qin Yujie;Ren Hui;Zhu Jianbo(College of Life Science,Shihezi University,Shihezi,832000)

机构地区：[1]石河子大学生命科学学院,石河子832000

出　　处：《分子植物育种》2024年第5期1430-1443,共14页Molecular Plant Breeding

基　　金：兵团科技攻关项目(2020AB009)资助。

摘　　要：中国是甜瓜的主要生产国家,种植面积和产量都位于世界首位。本研究通过全基因组分析鉴定了甜瓜MADS-box基因家族成员,分析甜瓜MADS-box基因家族成员的染色体定位、多重序列比对、motif分布和外显子-内含子结构分布,以及对其成员进行进化及表达分析,并分析启动子区的顺式作用元件分布。结果显示,从甜瓜全基因组数据库中共鉴定了45个甜瓜MADS-box基因,其中Ⅰ型基因11个,Ⅱ型基因34个;通过系统发育分析,Ⅰ型基因主要分布于Mβ和Mγ亚家族中;Ⅱ型基因则分布于13个亚家族;Ⅰ型基因与Ⅱ型基因的motif分布和外显子个数区别明显,但同型基因区别不大;对CmMADS-box基因家族全生理期表达量进行可视化分析并进行了共线性分析;根据启动子区的顺式作用元件分布情况,推测CmMADS-box基因可能响应多种外界胁迫,即具有功能多样性。该研究结果为探究甜瓜MADS-box基因家族生物学功能提供参考。China,in which lies the world's biggest planting area,becoming the main producer of muskmelon and the yield ranks first.Based on genome-wide analysis,we identified the members of MADS-box gene family in melon.The chromosomal location,multiple sequence alignment,motif distribution,exon-intron structure,phylogeny,expression pattern and cis-acting elements distribution of MADS-box gene family members were analyzed.The results showed that 45 CmMADS-box genes were identified from the whole genome database of melon,among which there are 11 of typeⅠgenes and 34 of typeⅡgenes.According to phylogenetic analysis,typeⅠgenes are mainly distributed in Mβand Mγsubfamilies and typeⅡgenes are distributed in 13 subfamilies.The motif distribution and the number of exons of typeⅠgene and typeⅡgene are different,but the difference of homotype genes is not significant.The expression of CmMADS-box gene family was performed visual analysisduring the whole physiological period.According to the prediction of cis-acting elements in the promoter region,the CmMADS-box gene may respond to a variety of environmental stresses,namely,it has functional diversity.The results of this study provide a reference for further exploring the biological functions of the MADS-box gene family in melons.

关 键 词：MADS-BOX 甜瓜 基因家族 进化分析 表达分析 

分 类 号：S652[农业科学—果树学]