河南省商城县2种动物发热伴血小板减少综合征病毒感染情况的调查研究  

Investigation on infection of severe fever with thrombocytopenia syndrome virus in two animals in Shangcheng County,Henan Province

作　　者：刘万双 陈浩荣 代科 蒋宝贵 陈津津 田珅 吴家红 刘玮 方立群 LIU Wanshuang;CHEN Haorong;DAI Ke;JIANG Baogui;CHEN Jinjin;TIAN Shen;WU Jiahong;LIU Wei;FANG Liqun(Department of Epidemiology and Health Statistics,School of Public Health,Guizhou Medical University,Guiyang 550025,China;Department of Pharmaceutical Engineering,College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China;State Key Laboratory of Pathogen and Biosecurity,Beijing Institute of Microbiology and Epidemiology,Beijing 100071,China;Department of Epidemiology and Health Dtatistics,School of Public Health,Guangzhou Medical University,Guangzhou 551436,China)

机构地区：[1]贵州医科大学公共卫生学院流行病与卫生统计学系,贵阳550025 [2]北京化工大学生命科学与技术学院制药工程学系,北京100029 [3]军事医学研究院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071 [4]广州医科大学公共卫生学院流行病与卫生统计学系,广州511436

出　　处：《中华疾病控制杂志》2024年第1期83-91,共9页Chinese Journal of Disease Control & Prevention

基　　金：国家重点研发计划项目(2019YFC1200604)。

摘　　要：目的通过开展发热伴血小板减少综合征(severe fever with thrombocytopenia syndrome,SFTS)自然疫源地动物宿主感染新布尼亚病毒(severe fever with thrombocytopenia syndrome virus,SFTSV)的调查研究,明确SFTS自然疫源地SFTSV的传播风险与影响因素,为SFTS的针对性防控提供科学依据。方法采用卫星定位技术连续跟踪商城县邱湾村、平塘村和龙头桥村3个村庄部分村民家养狗日常活动轨迹2周并采集血清样本,使用间接荧光抗体试验(indirect immunofluorescence assay,IFA)法检测家养狗血清SFTSV⁃IgG抗体,使用logistic回归分析模型分析影响家养狗感染SFTSV的潜在环境影响因素;采用夹(笼)夜法和粘鼠板法对家养狗活动区域内的啮齿动物及小型哺乳动物种群进行密度调查,针对保守基因进行PCR种类鉴定,并采用荧光PCR和酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)方法检测啮齿动物及小型哺乳动物SFTSV感染情况。结果通过卫星定位共追踪26条家养狗的日常活动范围信息,采集37条家养狗52份血清,SFTSV⁃IgG抗体总阳性率为59.46%;共捕获啮齿动物及小型哺乳动物419只,优势种为黄胸鼠、小家鼠、黑线姬鼠,未有SFTSV阳性标本检出;家养狗活动中心离主干道路距离远和到过林地的频次多是感染SFTSV的危险因素,家养狗活动中心离林地距离远是感染SFTSV的保护因素。结论商城县家养狗SFTSV抗体阳性率较高,感染SFTSV与生态环境相关,建议进一步扩大家养动物的种类,开展SFTSV感染情况的研究;啮齿动物的SFTSV感染率低,在SFTSV自然循环过程中的作用需进一步调查。Objective To clarify the risk and influential factors of severe fever with thrombocytopenia syndrome virus(SFTSV)transmission in natural foci of severe fever with thrombocytopenia syndrome(SFTS)by conducting an investigation and research on the infection of animal hosts in natural foci of SFTS,so as to provide scientific evidence for targeted prevention and control of SFTS.Methods Satellite positioning technology was used to continuously track the daily activities of domestic dogs in Qiuwan,Pingtang and Longtouqiao villages in Shangcheng County during two weeks of monitoring window.Serum samples of dogs were collected and detected SFTSV⁃IgG antibody by using indirect immunofluorescence assay(IFA)method.Multivariable logistic regression model was applied to analyze the effect of environmental influential factors for infection with SFTSV in domestic dogs.The species of rodents and small mammals in the active area of domestic dogs were investigated by night trapping,night⁃cage and sticky board methods.The conserved genes were identified by PCR.The SFTSV infection was detected by real⁃time PCR and enzyme linked immunosorbent assay(ELISA).Results The daily activity range of 26 domestic dogs was tracked by satellite positioning technology,and 52 serum samples were collected from 37 domestic dogs.The total positive rate of SFTSV antibody was 59.46%.A total of 419 rodents and small mammals were captured,and the dominant rat species were Rattus tanezumi,Mus musculus,and Apodemus agrarius,and no positive samples were detected for SFTSV.The longer distance from the domestic dog activity center to the main road and the higher frequency of visiting forest land were the risk factors for SFTSV infection.The longer distance from the domestic dog activity center to the forest land was the protective factor.Conclusions The positive rate of SFTSV antibody in domestic dogs in Shangcheng County is high,and the infection of SFTSV is related to the ecological environment.It is recommended to further expand the species of domestic

关 键 词：新布尼亚病毒 蜱传疾病 啮齿动物 血清学 危险因素 血小板减少综合征 发热 

分 类 号：R183.5[医药卫生—流行病学]