miR-20b-5p靶向VEGFA对深Ⅱ度烫伤创面组织的作用机制  

Mechanism of miR-20b-5p targeting VEGFA on deep second-degree burn wound healing

作　　者：王燕[1] 郭蕊 WANG Yan;GUO Rui(Department of Burn Surgery,the Third Affiliated Hospital of Inner Mongolia Medical University,Baotou 014010,China;Ordos Institute of Applied Technology,Ordos 017000,China)

机构地区：[1]内蒙古医科大学第三附属医院(内蒙古包钢医院)烧伤外科,内蒙古包头014010 [2]鄂尔多斯应用技术学院,内蒙古鄂尔多斯017000

出　　处：《临床皮肤科杂志》2024年第3期151-156,共6页Journal of Clinical Dermatology

摘　　要：目的:探究微小RNA-20b-5p(miR-20b-5p)对深Ⅱ度烫伤大鼠创面愈合的影响机制,以及其对血管内皮生长因子(vascular endothelial growth factor,VEGF)A的表达的调控机制。方法:沸水浴烙铁术制作深Ⅱ度烫伤大鼠模型,以miR-20b-5p抑制剂(miR-20b-5p-antagomir)干预创面组织,以miR-20b-5p-antagomir+小干扰RNA(small interfering RNA,siRNA)抑制VEGFA进行功能挽救;造模28 d后,检测大鼠的创面愈合率、创面组织中的胶原纤维含量以及血管新生。以miR-20b-5p-antagomir和miR-20b-5p-antagomir+siRNA-VEGFA分别转染微血管内皮细胞(human microvascular endothelial cells,HMEC)-1;检测细胞的增殖及小管形成的能力;Western blot检测各组创面组织和细胞中VEGFA的表达。结果:miR-20b-5p-antagomir能明显促进创面愈合,促进HMEC-1细胞的增殖与小管形成,而siRNA-VEGFA可部分逆转这一作用,差异均具有统计意义(P均<0.05)。结论:miR-20b-5p靶向VEGFA的表达影响深Ⅱ度烫伤大鼠的创面愈合,抑制miR-20b-5p的表达能促进创面愈合,促进血管新生。Objective:To explore the mechanism of microRNA-20b-5p(miR-20b-5p)on rats with deep second-degree burn wounds,and its regulatory mechanism on the expression of vascular endothelial growth factor A(VEGFA).Methods:Boiling water bath with a soldering iron was used to replicate a rat deep second-degree burn model.A miR-20b-5p inhibitor(miR-20b-5p antagomir)was used to intervene the wound tissue,and miR-20b-5p antagomir+small interfering RNA(small interfering RNA,siRNA)was used to inhibit VEGFA for functional rescue.After 28 days,the wound healing rate,collagen fiber content in the wound tissue,and the angiogenesis of rats were detected.With the experiments using HMEC-1 cells,miR-20b-5p antagonist and miR-20b-5p antagomir+siRNA VEGFA were transfected into HMEC-1 cells,respectively.The rate of cellular proliferation and the ability of HMEC-1 cells to form tubules were detected.Western blot was used to measure the expression of VEGFA in wound tissues and in HMEC-1 cells.Results:The miR-20b-5p-antagomir significantly promotes wound healing,as well as the proliferation and tubular formation of HMEC-1 cell,while siRNA VEGFA could partially reverse this effect.The differences were statistically significant(all P<0.05).Conclusion:A miR-20b-5p targeting the expression of VEGFA affects the wound healing of rats with deep second-degree burns.Inhibiting the expression of miR-20b-5p can promote wound healing and promote angiogenesis.

关 键 词：微小RNA-20b-5p 深Ⅱ度烫伤大鼠 血管内皮生长因子A 血管新生 

分 类 号：R644[医药卫生—外科学]