雾化吸入给药BD-77对呼吸道合胞病毒感染及LPS致小鼠肺炎的治疗作用  

Effect of Aerosol Inhalation Administration of BD⁃77 on Pneumonia Induced by Respiratory Syncytial Virus and Lipopolysaccharide in Mice

作　　者：孙静[1] 赵荣华[1] 孙建辉[1] 郭姗姗[1] 高双荣[1] 包蕾[1] 耿子涵 李舒冉 孙绮悦 徐洲 潘玉杰 陈运琴 孙田甜 陈红羽 黄成钢 崔晓兰[1] SUN Jing;ZHAO Ronghua;SUN Jianhui;GUO Shanshan;GAO Shuangrong;BAO Lei;GENG Zihan;LI Shuran;SUN Qiyue;XU Zhou;PAN Yujie;CHEN Yunqin;SUN Tiantian;CHEN Hongyu;HUANG Chenggang;CUI Xiaolan(Institute of Chinese Material Medical,China Academy of Chinese Medical Sciences,Beijing 100700,China;Shanghai Institute of Material Medical,Chinese Academy of Sciences,Shanghai 201203,China;Guizhou Bailing Enterprise Group Pharmaceutical Co.,LTD.,Guizhou 561099,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中国科学院上海药物研究所,上海201203 [3]贵州百灵企业集团制药股份有限公司,贵州561099

出　　处：《病毒学报》2024年第1期45-50,共6页Chinese Journal of Virology

基　　金：国家自然科学基金项目(项目号:82104500),题目:PINK-1/Parkin调节NLRP3炎症小体过度激活在冠状病毒性肺炎中的作用及马鞭草苷的干预研究。

摘　　要：评价BD‐77雾化吸入给药对呼吸道合胞病毒(Respiratory syncytial virus,RSV)及脂多糖(Lipopolysaccharide,LPS)致小鼠肺炎的治疗作用,为BD‐77的进一步开发提供实验依据。建立RSV及LPS滴鼻感染致小鼠肺炎模型,采用肺指数、肺指数抑制率、病毒载量、肺组织炎症因子等指标评价BD‐77的药理作用。BD‐77雾化吸入给药75 mg·mL^(‐1)、37.5 mg·mL^(‐1)两个剂量组可显著降低RSV感染小鼠肺指数、病毒载量和炎症因子释放。BD‐77静脉注射150 mg/(kg·d)、75 mg/(kg·d)两个剂量组对LPS引起的小鼠肺炎具有保护作用。BD‐77对RSV及LPS致小鼠肺炎均具有显著的保护作用。To study the protective effect of BD‐77 on pneumonia induced by respiratory syncytial virus(RSV)and lipopolysaccharide(LPS)in mice,and to provide experimental basis for further development of BD‐77.A mouse model of pneumonia induced by RSV and LPS was established by nasal drops.The pharmacological effects of BD‐77 were evaluated by lung index,lung index inhibition rate,viral load and inflammatory factors of lung tissue.Aerosol administration of BD‐77 at 75 mg·mL^(‐1) and 37.5 mg·mL^(‐1) for 25min could significantly reduce lung index,viral load and inflammatory factor release of RSV‐infected mice.Intravenous injection of 150 mg/(kg·d)and 75 mg/(kg·d)of BD‐77 had protective effects on lipopolysaccharide(LPS)induced pneumonia in mice.BD‐77 has significant protective effect on RSV and LPS induced pneumonia in mice.

关 键 词：BD-77 呼吸道合胞病毒 雾化吸入 肺炎 脂多糖 

分 类 号：R373.1[医药卫生—病原生物学]