广西桂林市急性呼吸道感染住院儿童人博卡病毒流行特征及基因特征分析  

Epidemiological and Genetic Characteristics of Human Bocavirus in Hospitalized Children with Acute Respiratory Tract Infection Cases in Guilin,Guangxi,China

作　　者：朱人和 王璐璐 程迁 宫悦 宗可鑫 宋娟[1] 宋芹芹[1] 梅国勇 夏志强 韩俊[1] 高晨[1] ZHU Renhe;WANG Lulu;CHENG Qian;GONG Yue;ZONG Kexin;SONG Juan;SONG Qinqin;MEI Guoyong;XIA Zhiqiang;HAN Jun;GAO Chen(National Institute for Viral Disease Control and Prevention,Chinese Centers for Disease Control and Prevention,National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,Beijing 102206,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,传染病溯源预警与智能决策全国重点实验室,北京102206

出　　处：《病毒学报》2024年第1期80-87,共8页Chinese Journal of Virology

基　　金：国家科技资源共享服务平台(项目号:NPRC-32),题目:国家病原微生物资源库;传染病预防控制国家重点实验室发展基金(项目号:2011SKLIDl04),题目:肠道病毒EV71通过先天性免疫致组织损伤机制研究;国家重点研发计划资助(项目号:2023YFC2308204),题目:基于“整体观”的复方中药抗病毒感染性疾病中的作用机理研究。

摘　　要：本研究旨在分析广西壮族自治区桂林市急性呼吸道感染住院儿童病例中人博卡病毒(Human bocavirus,HBoV)的流行特征及病毒基因特征。采用实时荧光定量聚合酶链式反应(qPCR)对2021年9月至2022年10月收集的638名因急性呼吸道感染住院儿童的鼻咽拭子样本进行了15种常见呼吸道病毒的检测。对HBoV核酸阳性的样本进行VP1基因巢式PCR扩增和序列测定,并与不同国家和地区HBoV1的VP1序列进行病毒基因特征分析。结果表明,在638例ARI病例样本中,有50例(7.84%)为HBoV阳性,96%(48/50)HBoV阳性病例均为5岁以下的儿童,其中以1~2岁患儿的占比最高(40%,20/50)。HBoV阳性病例主要集中在夏、秋、冬3个季节,但三者间差异无统计学意义(P>0.05)。在50例HBoV阳性病例中有29例(58.00%)为混合感染病例,HBoV的单一感染和混合感染均可引起上呼吸道或下呼吸道感染,但差异无统计学意义(P>0.05)。本研究所获得的16条HBoV序列均属于HBoV1,Ⅰa和Ⅰc分支中各分布有1条,其余14条均属于Ⅰb分支。HBoV是2021-2022年桂林地区导致儿童,尤其是2岁以下的婴幼儿呼吸道感染住院的主要病原体之一,存在Ⅰa、Ⅰb、Ⅰc三种型别共同流行的特点,其中Ⅰb分支为2021-2022年广西桂林市HBoV的主要优势流行株。The objective of this study is to delve into the epidemiological and genetic characteristics of Human Boca virus(HBoV)among hospitalized children suffering from acute respiratory infections in Guilin City,Guangxi,China.We employed real‐time fluorescence quantitative polymerase chain reaction(qPCR)to detect 15 common respiratory viruses in nasopharyngeal swab samples collected from 638 hospitalized children with acute respiratory infections over the period from September 2021 to October 2022.We conducted nested PCR amplification and sequencing of VP1 gene on HBoV nucleic acid‐positive samples.Additionally,we analyzed viral gene characteristics by comparing VP1 sequences of HBoV1 from diverse countries and regions.The results indicated that 50 cases(7.84%)out of 638 acute respiratory infection case samples tested positive for HBoV.Notably,96%(48/50)of HBoV‐positive cases were children under the age of 5,with the proportion of children aged 1‐2 years being the highest at 40%(20/50).HBoV‐positive cases were predominantly observed during summer,autumn and winter.However,no significant difference was observed among these three seasons(P>0.05).Among the 50 HBoV‐positive cases,29 cases(58.00%)were found to be mixed infections.Both single and mixed infections of HBoV can lead to upper or lower respiratory tract infections;however,the difference was not statistically significant(P>0.05).The 16 HBoV sequences obtained in this study all belonged to HBoV1.There was one sequence distributed in Ia and Ic branches,respectively,while the remaining 14 sequences belonged to Ib branch.HBoV is one of the primary pathogens responsible for respiratory tract infections in children,particularly infants and young children under 2 years old between 2021 and 2022 in the Guilin area,China.Notably,it exhibits common prevalence of three types:Ia,Ib and Ic.Among these,the Ib branch emerged as the dominant strain of HBoV between 2021 and 2022 in Guilin,Guangxi,China.

关 键 词：急性呼吸道感染 人博卡病毒 基因特征 

分 类 号：R373.9[医药卫生—病原生物学]