免疫PCR技术在微量非核酸分子检测方面的应用进展  

Research Progress on the Application of Immuno⁃PCR Technology in the Detection of Trace Non⁃nucleic Acid Molecules

作　　者：赵萌 申辛欣[5] 冯志山 ZHAO Meng;SHEN Xinxin;FENG Zhishan(Graduate School of Hebei Medical University,Shijiazhuang 050017,China;Hebei General Hospital,Shijiazhuang 050051,China;Hebei Key Laboratory of Molecular Medicine,Shijiazhuang 050051,China;Hebei Clinical Research Center for Laboratory Medicine,Shijiazhuang 050051,China;NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]河北医科大学研究生学院,石家庄050017 [2]河北省人民医院,石家庄050051 [3]河北省分子医学重点实验室,石家庄050051 [4]河北省医学检验临床医学研究中心,石家庄050051 [5]中国疾病预防控制中心病毒病预防控制所卫健委医学病毒与病毒病重点实验室,北京102206

出　　处：《病毒学报》2024年第1期197-205,共9页Chinese Journal of Virology

基　　金：国家重点研发计划(项目号:2021YFC2301100),题目:病原多场景实时检测关键技术平台的建立和应用;国家自然科学基金(项目号:82202593),题目:重组酶介导的核酸等温扩增(RAA)检测体系的优化及其在新型冠状病毒和流感病毒A/B型检测中的应用。

摘　　要：免疫PCR(Immuno-PCR,IPCR)技术是一种结合了酶联免疫吸附测定(Enzyme linked immunosorbent assay,ELISA)的特异性和聚合酶链式反应(Polymerase chain reaction,PCR)的高灵敏度的检测方法。与ELISA相比,IPCR由于PCR技术指数扩增的能力,检测灵敏度可以达到fg/mL。由于具有高灵敏度,IPCR在微量非核酸分子检测方面有着广泛的应用前景。本文主要介绍了传统IPCR和改良IPCR的原理和优缺点,概述了IPCR技术在病毒、细菌、寄生虫、肿瘤标志物、真菌、食品等方面的应用进展,总结了影响IPCR技术检测的因素并展望了IPCR技术的发展趋势。Immuno‐polymerase chain reaction(IPCR)is a method that combines the specificity of enzyme‐linked immunosorbent assay(ELISA)and the high sensitivity of polymerase chain reaction(PCR).Compared with ELISA,the sensitivity of IPCR can reach fg/mL due to the exponential amplification ability of PCR technology.Due to its high sensitivity,IPCR has a wide range of applications in detection of trace biomolecules.This review introduces the development process of IPCR,various forms of IPCR,and application of IPCR in virus,bacteria,parasites,tumor markers,fungi,environmental detection,etc.,all of these prove that IPCR technology has high application value in the detection of trace biomolecules.

关 键 词：IPCR ELISA PCR 标记DNA 捕获 免疫 

分 类 号：R373.9[医药卫生—病原生物学]