牛蒡子苷元通过调节Hippo-YAP信号通路对宫颈癌SiHa细胞增殖、迁移和侵袭的影响  

Effect of Arctigenin on the Proliferation,Migration,and Invasion of Cervical Cancer SiHa Cells by Regulating the Hippo-YAP Signaling Pathway

作　　者：姬国凤 崔红英 刘玉瑰 Ji Guofeng;Cui Hongying;Liu Yugui(Department of Obstetrics and Gynecology,The People's Hospital of Hebi,Hebi 458030,China)

机构地区：[1]鹤壁市人民医院妇产科,鹤壁458030

出　　处：《成都医学院学报》2024年第1期8-13,共6页Journal of Chengdu Medical College

基　　金：河南省医学科技攻关计划项目(No:LHGJ20200849)。

摘　　要：目的研究牛蒡子苷元调节Hippo-YAP信号通路对宫颈癌SiHa细胞增殖、迁移和侵袭的影响。方法将人子宫颈鳞癌细胞SiHa分为对照组、低浓度牛蒡子苷元组(5.0μmol/L)、中浓度牛蒡子苷元组(10.0μmol/L)、高浓度牛蒡子苷元组(20.0μmol/L)和高浓度牛蒡子苷元(20.0μmol/L)+TDI-011536组(3μmol/LHippo信号通路抑制剂)。分组处理后,集落形成实验检测细胞活性;Transwell实验检测细胞侵袭;划痕实验检测细胞迁移;免疫荧光技术检测细胞中Vimentin和E-Cadherin;蛋白质免疫印迹技术检测p-YAP、YAP、PCNA、MMP-2蛋白表达。结果与对照组比较,低浓度牛蒡子苷元组、中浓度牛蒡子苷元组、高浓度牛蒡子苷元组SiHa的细胞存活率、细胞侵袭数、细胞迁移愈合率、细胞中Vimentin荧光强度、YAP、PCNA、MMP-2蛋白表达均降低,而E-Cadherin荧光强度和p-YAP蛋白表达升高(P<0.05);与高浓度牛蒡子苷元组比较,高浓度牛蒡子苷元+TDI-011536组SiHa细胞存活率、细胞侵袭数、细胞迁移愈合率、细胞中Vimentin荧光强度、YAP、PCNA、MMP-2蛋白表达升高,而E-Cadherin荧光强度和p-YAP蛋白表达降低(P<0.05)。结论牛蒡子苷元可能通过激活Hippo/YAP信号通路抑制宫颈癌SiHa细胞的增殖、迁移和侵袭。Objective To investigate the effect of arctigenin on the proliferation,migration,and invasion of cervical cancer SiHa cells by regulating the Hippo-YAP signaling pathway.Methods Human cervical squamous cell carcinoma cells SiHa were grouped into three groups:control group,low concentration arctigenin group(5.0μmol/L),medium concentration arctigenin group(10.0μmol/L),high concentration arctigenin group(20.0μmol/L),and high concentration arctigenin group(20.0μmol/L)+TDI-011536 group(3μmol/L Hippo signaling pathway inhibitor).After grouping,colony formation experiment was applied to detect cell activity;Transwell was applied to detect cell invasion;Wound healing experiment was applied to detect cell migration;Immunofluorescence was applied to detect Vimentin and E-Cadherin in cells;Western blot was applied to detect the expression of p-YAP,YAP,PCNA,and MMP-2 proteins.Results Compared with the control group,the cell survival rate,cell invasion number,cell migration and healing rate,Vimentin fluorescence intensity,protein expression of YAP,PCNA,and MMP-2 of SiHa in low concentration,medium concentration,and high concentration arctigenin groups decreased,while E-Cadherin fluorescence intensity and the expression of p-YAP protein increased(P<0.05).Compared with the high concentration arctigenin group,the cell survival rate,cell invasion number,cell migration and healing rate,Vimentin fluorescence intensity,protein expression of YAP,PCNA,and MMP-2 of SiHa in the high concentration arctigenin+TDI-011536 group increased,while E-Cadherin fluorescence intensity and the expression of p-YAP protein decreased(P<0.05).Conclusion Arctigenin may inhibit the proliferation,migration,and invasion of cervical cancer SiHa cells by activating the Hippo/YAP signaling pathway.

关 键 词：牛蒡子苷元 Hippo-YAP信号通路 宫颈癌 增殖 迁移和侵袭 

分 类 号：R285[医药卫生—中药学]