近江蛏溶质载体6(SLC6)基因家族的鉴定及其在盐胁迫下的表达分析  

Identification of Solute Carrier 6(SLC6)Gene Family of Sinonovacula rivularis and Its Expression Analysis Under Salinity Stress

作　　者：王莎莎 姚韩韩 何琳 何京[2] 董迎辉[2] 林志华[2] WANG Sha-Sha;YAO Han-Han;HE Lin;HE Jing;DONG Ying-Hui;LIN Zhi-Hua(College of Biological&Environmental Sciences,Key Laboratory of Aquatic Germplasm Resource of Zhejiang,Zhejiang Wanli University,Ningbo 315100,China;Ninghai Institute of Mariculture Breeding and Seed Industry,Zhejiang Wanli University,Ninghai 315604,China)

机构地区：[1]浙江万里学院生物与环境学院/浙江省水产种质资源高效利用技术研究重点实验室,宁波315100 [2]浙江万里学院宁海海洋生物种业研究院,宁海315604

出　　处：《农业生物技术学报》2024年第3期628-640,共13页Journal of Agricultural Biotechnology

基　　金：国家现代贝类产业技术体系(CARS-49);宁波市重大科技攻关暨“揭榜挂帅”项目(2021Z114)。

摘　　要：膜转运蛋白的溶质载体6(solute carrier 6,SLC6)基因家族被称为神经递质转运体,在水生生物应激胁迫调控、渗透调节中发挥着重要作用。本研究对近江蛏(Sinonovacula rivularis)SLC6基因家族成员(SrSLC6)进行了鉴定和生物信息学分析,并分析了其在盐胁迫下的表达模式。结果表明,近江蛏共有29个SrSLC6基因家族成员,分属于4个亚家族,具有12个保守基序和2个保守结构域SLC6sbd-TauT-like和SLC5-6-like_sbd;在高、低盐度胁迫下,16个SrSLC6基因呈现不同的表达响应,表达量随盐度变化呈现不同程度的上调或下调;与盐度适应相关的近江蛏氨基酸转运体4(amino acid transporter 4 of S.rivularis,SrAAT4)蛋白主要定位在鳃中鳃丝侧面的侧纤毛柱状细胞和扁平细胞,表达模式与转录组表达谱趋势相同,即在低盐胁迫不同时间点表达量均显著下降(P<0.05),在高盐胁迫不同时间点表达量均显著升高(P<0.05)。综上结果表明,SrSLC6基因家族成员氨基酸转运体(amino acid transporters,AATs)和神经递质转运体(neurotransmitter transporters,NTTs)可能在近江蛏渗透压调节中发挥关键作用。本研究为深入开展SLC6基因家族在双壳贝类渗透压调节和盐度适应研究提供理论依据。The solute carrier 6(SLC6)gene family,known as neurotransmitter transporters,plays a crucial role in stress regulation and osmotic regulation of aquatic organisms.In the present study,the members of the SLC6 gene family of Sinonovacula rivularis(SrSLC6)were identified and analyzed by using bioinformatics methods,and expression patterns under salinity stress were investigated.The results showed that a total of 29 SrSLC6 genes were identified,which belonged to 4 subfamilies and contained 12 conserved motifs and 2 conserved domains SLC6sbd-TauT-like and SLC5-6-like_sbd.Under high and low salinity stress,16 SrSLC6 gene family members showed different expression responses and their expression levels were upregulated or down-regulated.Amino acid transporter 4 protein of S.rivularis(SrAAT4)related to salinity adaptation was mainly located in lateral ciliated columnar cells and flat cells on the side of gill filaments,and the expression patterns showed the same trend as transcriptome expression profile,which was significantly decreased at different time points under low salt stress(P<0.05),and significantly increased at different time points under high salt stress(P<0.05).These results indicated that the amino acid transporters(AATs)and neurotransmitter transporters(NTTs)members of SrSLC6 gene family might played important roles in osmotic regulation of S.rivularis,which provided theoretical basis for further research on the osmotic regulation and salinity adaptation of SLC6 gene family in mollusks.

关 键 词：近江蛏 溶质载体6(SLC6) 基因家族 盐胁迫 

分 类 号：S917[农业科学—水产科学]