鼻咽癌组织中GPX4和SLC7A11的表达及临床意义  

Expression and clinical significance of GPX4 and SLC7A11 in nasopharyngeal carcinoma

作　　者：王春艳 赵兴泉[1] 徐鑫 WANG Chunyan;ZHAO Xingquan;XU Xin(Department of Otorhinolaryngology,Sichuan Mianyang 404 Hospital/Mianyang First People′s Hospital,Mianyang,Sichuan 621000,China)

机构地区：[1]四川绵阳四0四医院/绵阳市第一人民医院耳鼻咽喉科,四川绵阳621000

出　　处：《国际检验医学杂志》2024年第5期517-522,共6页International Journal of Laboratory Medicine

基　　金：四川省卫生健康委员会适宜技术推广项目(19SYJS15)。

摘　　要：目的探讨鼻咽癌(NPC)组织中谷胱甘肽过氧化物酶4(GPX4)、溶质载体家族7成员11(SLC7A11)的表达及临床意义。方法将2016年3月至2017年3月于本院诊治的98例NPC患者纳入研究作为NPC组,同期因鼻中隔偏曲接受手术治疗的患者作为对照组。采用免疫组化检测NPC组织和正常鼻黏膜组织中GPX4、SLC7A11的表达情况。采用Spearman秩相关分析癌组织中GPX4、SLC7A11表达的相关性。比较不同NPC临床参数患者之间GPX4、SLC7A11表达阳性率。采用Kaplan-Meier法分析GPX4、SLC7A11表达对NPC患者生存预后的影响。采用单因素及多因素Cox回归分析NPC患者生存预后的影响因素。结果NPC组织中GPX4、SLC7A11表达阳性率分别为75.51%(74/98)、73.47%(72/98),分别高于正常鼻黏膜组织中的11.67%(7/60)、13.33%(8/60),差异均有统计学意义(P<0.001)。NPC组织中GPX4与SLC7A11表达呈正相关(r=0.724,P<0.001)。不同临床分期、肿瘤分化程度、淋巴结转移情况及放疗敏感性NPC患者癌组织中GPX4、SLC7A11表达阳性率比较,差异均有统计学意义(P<0.05)。GPX4阳性及阴性表达组的5年总体生存率分别为66.22%(49/74)、91.67%(22/24);GPX4阳性表达组累积生存明显低于阴性表达组(Log-rankχ^(2)=5.822,P<0.001)。SLC7A11阳性及阴性表达组5年总体生存率分别为66.67%(48/72)、88.46%(23/26);SLC7A11阳性表达组累积生存明显低于阴性表达组(Log-rankχ^(2)=5.041,P=0.012)。临床分期Ⅲ~Ⅳ期(HR=1.608,95%CI:1.225~2.112)、淋巴结转移(HR=1.917,95%CI:1.319~2.799)、GPX4阳性(HR=1.839,95%CI:1.228~2.753)、SLC7A11阳性(HR=1.738,95%CI:1.246~2.426)是影响NPC患者生存预后的独立危险因素。结论NPC中GPX4、SLC7A11表达水平升高,两者与不良临床病理特征有关,是NPC患者预后评估的潜在标志物。Objective To investigate the expression and clinical significance of glutathione peroxidase 4(GPX4)and solute carrier family 7 member 11(SLC7A11)in nasopharyngeal carcinoma(NPC)tissues.Methods Ninety-eight NPC patients who were treated in the hospital from March 2016 to March 2017 were enrolled in the study as the NPC group,and the patients who underwent surgical treatment for nasal septal deviation during the same period were enrolled as the control group.Immunohistochemistry was used to detect the expression of GPX4 and SLC7A11 proteins in NPC tissue and normal nasal mucosa tissue.Spearman rank correlation was used to analyze the correlation of GPX4 and SLC7A11 protein expression in cancer tissue.The positive rates of GPX4,SLC7A11 protein expression between patients with different NPC clinical parameters were compared.The influnces of GPX4,SLC7A11 expression on the survival prognosis of NPC patients were analyzed by using Kaplan-Meier method.The influencing factors of survival prognosis of NPC patients were analyzed by using unifactorial and multifactorial Cox regression.Results The positive expression rates of GPX4 and SLC7A11 in NPC tissue were 75.51%(74/98)and 73.47%(72/98),respectively,which were higher than 11.67%(7/60)and 13.33%(8/60)in normal nasal mucosa tissue,and the differences were statistically significant(P<0.001).GPX4 expression was positively correlated with SLC7A11 expression in NPC tissues(r=0.724,P<0.001).There were significant differences in the positive expression rates of GPX4 and SLC7A11 in NPC patients with different clinical stages,tumor differentiation,lymph node metastasis state and radiotherapy sensitivity(P<0.05).The 5-year overall survival rates of GPX4 positive and negative expression groups were 66.22%(49/74)and 91.67%(22/24),respectively;the cumulative survival of GPX4 positive expression group was significantly lower than that of GPX4 negative expression group(Log-rankχ^(2)=5.822,P<0.001).The 5-year overall survival rates of SLC7A11 positive and negative expression groups were

关 键 词：鼻咽癌 谷胱甘肽过氧化物酶4 溶质载体家族7成员11 预后 

分 类 号：R446.8[医药卫生—诊断学] R739.63[医药卫生—临床医学]