血清IL-7和Hcy水平与胃癌患者Lauren分型及病灶内上皮间质转化的关系  

The relationship between changes in serum IL-7 and Hcy expression and Lauren classification and intralesional epithelial interstitial transformation in gastric cancer patients

作　　者：宫向良 刘征 徐爱芳 李世龙[2] 丁梅 宋扬[2] GONG Xiangliang;LIU Zheng;XU Aifang;LI Shilong;DING Mei;SONG Yang(Department of Endoscopic Surgery,Tangshan Central Hospital,Tangshan,Hebei 063000,China;Department of Clinical Laboratory,Tangshan People′s Hospital,Tangshan,Hebei 063000,China)

机构地区：[1]唐山中心医院腔镜外科,河北唐山063000 [2]唐山市人民医院检验科,河北唐山063000

出　　处：《国际检验医学杂志》2024年第5期569-572,577,共5页International Journal of Laboratory Medicine

基　　金：河北省2018年度医学科学研究重点课题计划项目(20181244)。

摘　　要：目的探讨血清白细胞介素(IL)-17和同型半胱氨酸(Hcy)水平与胃癌患者Lauren分型及病灶内上皮间质转化(EMT)的关系。方法将唐山中心医院2021年5月至2023年5月收治的原发性胃癌患者78例纳入研究作为胃癌组。另外,选取同期于唐山中心医院就诊及治疗的78例胃良性病变者作为良性病变组,选取同期于唐山中心医院体检的健康者78例作为健康对照组。检测纳入研究者血清IL-7和Hcy水平。根据Lauren分型,将胃癌组患者进一步分为肠型胃癌组43例、弥漫型胃癌组35例。检测胃癌组和良性病变组病灶组织中的基质金属蛋白酶9(MMP-9)、VEGF、β-连接素(β-catenin)、上皮性钙黏附蛋白(E-Cadherin)、波形蛋白(Vimentin)、CD68、神经降压素(NTS)、IL-8等病灶内EMT相关指标并计算细胞染色阳性率(PR)。结果胃癌组血清IL-17、Hcy水平均高于良性病变组及健康对照组(P<0.05),良性病变组血清IL-17、Hcy水平均高于健康对照组(P<0.05)。胃癌组病灶组织NTS、IL-8、VEGF、MMP-9、CD68、β-catenin、E-Cadherin、Vimentin PR均高于良性病变组(P<0.05)。肠型胃癌组血清IL-17、Hcy水平均高于弥漫型胃癌组(P<0.05)。血清IL-7和Hcy水平与病灶组织NTS、IL-8、VEGF、MMP-9、CD68、β-catenin、E-Cadherin、Vimentin等EMT相关指标PR均呈正相关(P<0.05)。结论IL-17、Hcy在胃癌患者血清中水平较高,均与Lauren分型及病灶内EMT相关指标有关。Objective To investigate the relationship of serum interleukin(IL)-17 and homocysteine(Hcy)levels with Lauren classification and epithelial-mesenchymal transition(EMT)in gastric cancer patients.Methods A total of 78 patients with primary gastric cancer admitted to Tangshan Central Hospital from May 2021 to May 2023 were enrolled in the study as the gastric cancer group.In addition,78 patients with benign gastric lesions who were treated in Tangshan Central Hospital during the same period were selected as the benign lesion group,and 78 healthy people who underwent physical examination in Tangshan Central Hospital during the same period were selected as the healthy control group.The serum levels of IL-7 and Hcy were detected.According to Lauren classification,the gastric cancer group was further divided into intestinal-type gastric cancer group(43 cases)and diffuse-type gastric cancer group(35 cases).Matrix metalloproteinase-9(MMP-9),vascular endothelial growth factor(VEGF),β-catenin,E-Cadherin,Vimentin,CD68,neurotensin(NTS),IL-8 and other EMT-related indicators in the lesions of gastric cancer group and benign lesion group were detected and the positive rate of cell staining(PR)was calculated.Results The serum levels of IL-17 and Hcy in the gastric cancer group were higher than those in the benign lesion group and the healthy control group(P<0.05).The serum levels of IL-17 and Hcy in the benign lesion group were higher than those in the healthy control group(P<0.05).The PRs of NTS,IL-8,VEGF,MMP-9,CD68,β-catenin,E-Cadherin and Vimentin in the gastric cancer group were higher than those in the benign lesion group(P<0.05).The serum levels of IL-17 and Hcy in intestinal-type gastric cancer group were higher than those in diffuse-type gastric cancer group(P<0.05).The levels of serum IL-7 and Hcy were positively correlated with the PRs of EMT-related indicators such as NTS,IL-8,VEGF,MMP-9,CD68,β-catenin,E-Cadherin,Vimentin(P<0.05).Conclusion The serum levels of IL-17 and Hcy in patients with gastric cancer are higher

关 键 词：白细胞介素-17 同型半胱氨酸 胃癌 Lauren分型 上皮间质转化 

分 类 号：R446.11[医药卫生—诊断学]