BRAF、TP53、Pax8-PPARγ在甲状腺癌中的表达及疗效预测价值  

Expression of BRAF,TP53,and Pax8-PPARγin thyroid cancer and their predictive value for therapeutic efficacy

作　　者：苏镇军 赵艳春 李娟 国方娜 温丽莎[1] SU Zhenjun;ZHAO Yanchun;LI Juan;GUO Fangna;WEN Lisha(Department of Nuclear Medicine,Affiliated Hospital of Hebei Engineering University,Handan,Hebei 056002,China)

机构地区：[1]河北工程大学附属医院核医学科,河北邯郸056002

出　　处：《国际检验医学杂志》2024年第5期598-602,607,共6页International Journal of Laboratory Medicine

基　　金：邯郸市科学技术研究与发展计划项目(22422083084ZC)。

摘　　要：目的探讨肿瘤蛋白P53(TP53)、丝氨酸/苏氨酸蛋白激酶(BRAF)、配对盒基因8-过氧化物酶体增殖物激活受体γ(Pax8-PPARγ)在甲状腺癌中的表达及疗效预测价值。方法将2020年4月至2022年4月该院收治的150例甲状腺癌患者纳入研究。检测患者甲状腺癌组织及癌旁组织中TP53、BRAF、Pax8-PPARγmRNA的表达水平,分析其与临床病理因素的关系,基于受试者工作特征(ROC)曲线及决策曲线分析TP53、BRAF、Pax8-PPARγmRNA表达水平预测^(131)I治疗效果的价值。结果甲状腺癌组织中的TP53、BRAF、Pax8-PPARγmRNA表达水平高于癌旁组织(P<0.05)。甲状腺癌患者淋巴结转移、肿瘤最大径、包膜浸润与TP53、BRAF、Pax8-PPARγmRNA表达水平有关(P<0.05)。采用放射性^(131)I清除术后残留的甲状腺组织(简称清甲)失败患者组织TP53、BRAF、Pax8-PPARγmRNA表达水平均高于清甲成功患者(P<0.05)。ROC曲线分析显示,TP53、BRAF、Pax8-PPARγmRNA联合预测甲状腺癌患者清甲失败的曲线下面积优于三者单独预测(P<0.05)。决策曲线显示,三者联合预测甲状腺癌清甲失败发生的净收益率优于单一预测(P<0.05)。结论TP53、BRAF、Pax8-PPARγ在甲状腺癌组织中呈高表达,联合检测有助于预测^(131)I治疗效果,为临床确定合理治疗方案及时机提供参考。Objective To investigate the expression and prognostic value of tumor protein P53(TP53),serine/threonine protein kinase(BRAF),paired box gene 8 antibody-peroxisome proliferator-activated receptor gamma(Pax8-PPARγ)in thyroid cancer.Methods A total of 150 patients with thyroid cancer admitted to the hospital from April 2020 to April 2022 were enrolled in the study.The expression levels of TP53,BRAF and Pax8-PPARγmRNA in thyroid cancer tissues and adjacent tissues were detected,and their relationship with clinicopathological factors was analyzed.Receiver operating characteristic(ROC)curve and decision curve were used to analyze the value of TP53,BRAF,Pax8-PPARγmRNA expression levels in predicting the response to^(131)I treatment.Results The expression levels of TP53,BRAF,and Pax8-PPARγmRNA in thyroid cancer tissues were higher than those in paracancerous tissues(P<0.05).Lymph node metastasis,maximum tumor diameter,and peritumoral infiltration in thyroid cancer patients were associated with TP53,BRAF,and Pax8-PPARγmRNA expression levels(P<0.05).The expression levels of TP53,BRAF,and Pax8-PPARγmRNA in the tissues of patients who failed redioiodine remnent ablation were higher than those of patients who succeeded(P<0.05).The ROC curve analysis showed that the combination of TP53,BRAF,and Pax8-PPARγmRNA predicted the failure of to remove the residual thyroid tissues after redioiodine remnent ablation using radioactive^(131)I in thyroid cancer patients with larger area under the curve than those predicted by the three alone(P<0.05).The decision curve showed that the net return rate of the combined prediction of the three factors was better than that of the single prediction(P<0.05).Conclusion TP53,BRAF,and Pax8-PPARγare highly expressed in thyroid cancer tissue,and the combined detection can help predict the effect of^(131)I treatment and provide reference for clinical determination of reasonable treatment plan and time.

关 键 词：甲状腺癌 配对盒基因8-过氧化物酶体增殖物激活受体γ 丝氨酸/苏氨酸蛋白激酶 肿瘤蛋白P53 ^(131)I治疗 

分 类 号：R446.11[医药卫生—诊断学]