Pseudomonas Cyclic Lipopeptide Medpeptin:Biosynthesis and Modulation of Plant Immunity  

作　　者：Yi-Lin Gu Jun-Zhou Li Yan Li Shen Cong Jing Wang Yi-Nan Ma Hai-Lei Wei 

机构地区：[1]State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China,Key Laboratory of Microbial Resources Collection and Preservation,Ministry of Agriculture and Rural Affairs,Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《Engineering》2023年第9期153-165,共13页工程（英文）

基　　金：funded by the National Key R&D Program of China(2022YFD1901300);the National Natural Science Foundation of China(31901932);the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202308 and Y2022PT12);the Beijing Innovation Consortium of Agriculture Research System(BAIC04-2022);the Science and Technology Programs of the Zunyi Tobacco(2021XM03)。

摘　　要：The multifunctional secondary metabolites known as cyclic lipopeptides(CLPs),which are produced by a large variety of bacteria,have become a key category of plant immunity elicitors.Pseudomonas-CLPs(PsCLPs)are extremely diverse in structure and biological activity.However,an understanding of CLP-plant structure–function interactions currently remains elusive.Here,we identify medpeptin,a novel CLP from Pseudomonas mediterranea that consists of 22 amino acids.Medpeptin is synthesized by a non-ribosomal peptide synthase(NRPS)gene cluster and regulated by a quorum-sensing system.Further research indicates that medpeptin does not exhibit antimicrobial activity;instead,it induces plant cell death immunity and confers resistance to bacterial infection.Comparative transcriptome analysis and virus-induced gene silencing(VIGS)reveal a set of immune signaling candidates involved in medpeptin perception.Silencing of a cell-wall leucine-rich repeat extensin protein(NbLRX3)or a receptor-like protein kinase(NbRLK25)—but not BAK1 or SGT1—compromises medpeptin-triggered cell death and resistance to pathogen infection in Nicotiana benthamiana.Our findings point to a noncanonical mechanism of CLP sensing and suggest perspectives for the development of plant disease resistance.

关 键 词：PSEUDOMONAS Cyclic lipopeptide Cell death Leucine-rich repeat extension(LRX) Medpeptin Receptor-like kinase(RLK) 

分 类 号：S432.2[农业科学—植物病理学]