蛋白质结构稳定性检测的实验课程设计  

Design of the experimental course for thermostability determination of protein

作　　者：石玉华[1] 谢鹍鹏 崔帅 单亚明[1] Shi Yuhua;Xie Kunpeng;Cui Shuai;Shan Yaming(School of Life Sciences,Jilin University,Changchun 130012,China)

机构地区：[1]吉林大学生命科学学院,吉林长春130012

出　　处：《现代科学仪器》2024年第1期167-170,共4页Modern Scientific Instruments

基　　金：吉林省科学技术厅资助项目(20220204008YY)。

摘　　要：随着生物技术的突破和发展,多肽蛋白类药物的出现使得人类疾病的诊断和治疗发生了翻天覆地的变化。为了顺应多肽蛋白类药物的飞速发展,加强生命科学等专业本科生对此类药物制剂相关知识的普及已势在必行。此实验课程设计利用稳定性热检测法分析蛋白质结构的稳定性,为生命科学等专业本科生设计一堂深入了解蛋白质结构与功能关系的课程,以促进本科生拓宽学术视野,提升完成相关科学研究和适应医药产业发展新形势的能力。In the face of breakthroughs and advancements in biotechnology,the advent of polypeptide protein drugs has catalyzed a profound transformation in the diagnosis and treatment of human diseases.As these polypeptide protein drugs evolve rapidly,it is becoming increasingly essential to bolster knowledge related to these drug among undergraduate students,particularly those majoring in life sciences and associated fields.To cater to this need,we have architected a unique experimental course that incorporates heat stability testing of purified proteins or peptides as a central research topic.This experimental course is designed to utilize thermal stability assays for the analysis of protein structure stability,and is tailored for undergraduate students in life sciences and related majors.The course aims to provide an in-depth understanding of the relationship between protein structure and function,in order to broaden the academic horizons of undergraduate students,and enhance their capabilities in conducting relevant scientific research and adapting to the evolving trends in the pharmaceutical industry.

关 键 词：多肽蛋白类药物 蛋白质结构与功能 蛋白质结构稳定性 实验课程设计 生命科学专业 

分 类 号：Q-337[生物学]